Advantages of Supports Activated with Divinyl Sulfone in Enzyme Coimmobilization: Possibility of Multipoint Covalent Immobilization of the Most Stable Enzyme and Immobilization via Ion Exchange of the Least Stable Enzyme

Authors: Morellon-Sterling, R; Carballares, D; Arana-Pena, S; Siar, EH; Braham, SA; Fernandez-Lafuente, R

Article.
ACS Sustain. Chem. Eng.. vol: 9. page: 2168-0485.
Date: jun-07. 2021.
Doi: 10.1021/acssuschemeng.1c01065.

Abstract:
Vinyl sulfone (VS)-agarose beads were used to develop a new strategy to coimmobilize enzymes with very different stabilities, enabling the reuse of the most stable immobilized enzyme. Two model combi-biocatalysts were prepared. First, trypsin [read more]