Authors: Palomo, JM

Curr. Opin. Green Sustain. Chem.. vol: 29. page: 2452-2236.
Date: JUN. 2021.
Doi: 10.1016/j.cogsc.2021.100452.

Artificial enzymes based on the modification of a protein structure for the creation of new active catalytic sites have experienced a great boom in recent years. Multidisciplinary strategies of genetic engineering, chemical or chemical biological tools have been successfully described to synthetize them. However, a challenge has been focused on the creating of artificial enzymes with more than one active site. This could represent a new direction in the application of enzymatic tools in sustainable chemistry. Actually, only a few technologies have been described for designing artificial enzymes with two or multiple active sites. This review article underlines these most significant advances..