Switch off/switch on of a cysteinyl protease as a way to preserve the active catalytic group by modification with a reversible covalent thiol modifier: Immobilization of ficin on vinyl-sulfone activated supports

Autores: Morellon-Sterling, R; Bolivar, JM; Fernandez-Lafuente, R

Artículo.
Int. J. Biol. Macromol.. vol: 220. page: 0141-8130.
Fecha: nov-01. 2022.
Doi: 10.1016/j.ijbiomac.2022.08.155.

Resumen:
The immobilization of ficin (a cysteinyl proteases) on vinyl sulfone agarose produced its almost full inactivation. It was observed that the incubation of the free and immobilized enzyme in beta-mercaptoethanol produced a 20 % of enzyme activity recovery, suggesting that [read more]