Further Stabilization of Alcalase Immobilized on Glyoxyl Supports: Amination Plus Modification with Glutaraldehyde
Authors: Hussain, F; Arana-Pena, S; Morellon-Sterling, R; Barbosa, O; Braham, SA; Kamal, S; Fernandez-Lafuente, R
Molecules. vol: 23. page: 1420-3049.
Date: DEC. 2018.
Alcalase was immobilized on glyoxyl 4% CL agarose beads. This permitted to have Alcalase preparations with 50% activity retention versus Boc-l-alanine 4-nitrophenyl ester. However, the recovered activity versus casein was under 20% at 50 degrees [read more]