GARCÍA RUIZ EVA

Garcia Ruiz Eva
Teléfono: 917145861
Teléfono interno: 437513
Group: Evolución dirigida de enzimas
Dept: BIOCATÁLISIS
Despacho: ---
Lab: 305
Responsable: ALCALDE GALEOTE, MIGUEL

BIOGRAFÍA

Dra. Eva García obtained her B.Sc. in Biology in the Universidad de Alcala in 2004. During her studies she spent 2 years at the Prof. Jose Ramon de Lucas lab as research fellow learning molecular biology techniques. Later, she was hired by Merck, Sharp & Dohme (MSD) for 1.5 years under the supervision of Prof. Gerald Bills. During this period (2005-2006), she got familiarized with high-throughput biomolecular screening and automation environments as well as gained experience in the field of natural products with pharmaceutical purposes.

In 2006, she was recruited by Prof. Ángel T. Martínez and Dr. Miguel Alcalde (CSIC) to carried out her PhD Thesis with European mention and titled “Directed Evolution of a versatile peroxidase from Pleurotus eryngii in Saccharomyces cerevisiae” (unanimous Summa Cum Laude in Nov 2012). During her PhD she also spent a short stay at Prof. Rolang Ludwig’s Lab in University of Natural Resources and Applied Life Sciences (BOKU). This Thesis work was funded by three consecutive European Projects (with over 30 different European partners between Academia and Industry) that allowed Eva to participate in international meetings and prestigious scientific conferences where she defended her research. The main achievement of this work was the generation of a number of peroxidase variants with unique properties resulting in several international publications and a patent.

After obtaining the PhD, Eva Garcia continued working at Dr. Alcalde’s lab (pioneering group for the establishment of the Directed Evolution methodology in Spain) in an industrial project with Repsol SA where she led the research line of directed evolution of enzymes. This resulted in a patent filling and several international publications.

Subsequently, Eva joined the laboratory of Prof. Huimin Zhao at the University of Illinois at Urbana-Champaign, Illinois, USA, an international renown group in the field of Synthetic Biology. In this stage (2013-2016), she led two research lines for the design of multi-enzyme routes and genome editing using synthetic biology tools for the biofuel production in microorganisms and plants from alternative natural resources. During this period, she also participated in the establishment of the first “Living Foundry”, iBioFab (Illinois Biological Foundations for Advanced Biomanufacturing).

Afterwards, she was recruited by Prof. Patrick Yizhi Cai at the University of Edinburgh, Edinburgh, UK (2016), co-founder of the Edinburgh Genome Foundry and International Coordinator of the Consortium Sc2.0, consortium building the world's first synthetic eukaryotic genome. At this position, Eva García was leading the research line for the biosynthesis of natural products and the setup of the automation platform for the design, manufacture, validation and analysis of multi-enzyme pathways to produce natural compounds in yeast. She continued this research in the Manchester Institute of Biotechnology (MIB), Manchester, UK when Prof. Cai moved his lab to MIB in 2017.

Recently, Eva García was recruited by Dr. Alcalde to lead a research line to optimize multi-enzyme pathways for the bio-based production of isoprenoids (a family of compounds precursor of a variety of industrial relevant compounds).
The scientific track record of Eva García has been shaped towards the design and engineering of enzymes and multi-enzyme routes in organisms for their application in different fields of biotechnological interest. She has been working in pioneering and international renown research groups in synthetic biology and directed evolution learning the state-of-the-art of different tools of directed evolution, synthetic biology, metabolic engineering and genome edition, while specializing in automation of those tools.

PUBLICACIONES:

1. Auxillos, J.Y.; Garcia-Ruiz, E.; Jones, S.; Li, T.; Jiang, S.; Dai, J.; Cai, Y. (2019) Multiplex genome engineering for optimizing bioproduction in Saccharomyces cerevisiae. Biochemistry, DOI: 10.1021/acs.biochem.8b01086.

2. Garcia-Ruiz, E.; Auxillos, J.; Li, T.; Dai, J.; Cai, Y. (2018) YeastFab: High-throughput genetic parts construction, measurement, and pathway engineering in yeast. Methods in Enzymology, 608:277-306.

3. Martin-Diaz, J.; Paret, C.; Garcia-Ruiz, E.; Molina-Espeja, P.; Alcalde, A. (2018) Shuffling the neutral drift of unspecific peroxygenase in Saccharomyces cerevisiae. Applied and environmental microbiology, 84(15): e00808-18.

4. Gomez-Fernandez, BJ.; Garcia-Ruiz, E.; Martin-Diaz, J.; Gomez de Santos, P.; Santos-Moriano, P.; Plou, FJ.; Ballesteros, A.; Garcia, M.; Rodriguez, M.; Risso, VA.; Sanchez-Ruiz, JM.; Whitney, S; Alcalde, M.(2018) Directed -in vitro- evolution of Precambrian and extant Rubiscos. Scientific reports. 8(1), 5532.

5. Garcia-Ruiz, E., Badur, A., Rao, CV., and Zhao, H. (2016) Characterization and engineering of seaweed degrading enzymes for biofuels and biochemicals production. In Quality Living Through Chemurgy and Green Chemistry. Springer Berlin Heildelberg. 99-128. ISBN 978-3-662-53704-6.

6. Garcia-Ruiz, E., HamediRad, M., and Zhao, H. (2016) Pathway design, engineering and optimization. In Advances in Biochemical Engineering/Biotechnology. Springer Berlin Heildelberg. 1-40. ISSN 0724-6145.

7. Molina-Espeja, P., Viña-Gonzalez, J., Gomez, BJ., Martin-Diaz, J., Garcia-Ruiz, E., and Alcalde, M. (2016) Beyond the outer limits of nature by directed evolution. Biotechnology Advances. 34:754-767.

8. González-Pérez, D., Garcia-Ruiz, E., Ruiz-Dueñas, F.J., Martinez, A.T and Alcalde, M. (2014) Structural determinants of oxidative stabilization in an evolved versatile peroxidase. ACS Catalysis. 4:3891-3901.

9. Molina-Espeja, P., Garcia-Ruiz, E., González-Pérez, D., Ullrich, R., Hofrichter, M., and Alcalde, M. (2014) Directed evolution of unspecific peroxygenase from Agrocybe aegerita. Applied and environmental microbiology. 80: 3496-3507.

10. Garcia-Ruiz, E., González-Pérez, D., Ruiz-Dueñas, F.J., Martínez, A.T., Alcalde, M. (2012). Directed evolution of a temperature-, peroxide- and alkaline pH-tolerant versatile peroxidase. Biochemical Journal. 441: 487-98.

11. Garcia-Ruiz, E., Maté, D., Ballesteros, A., Martínez, A.T. and Alcalde M. (2010). Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast. Microbial Cell Factories. 9: 17-30.

Patents

1. Inventores (p.o. de firma): Rodríguez-Buey, M., Garcia-Ruiz, M., Martin-Diaz, J., Santos-Moriano, P.C., Molina-Espeja, M., Garcia-Ruiz, E., Plou, F.J., and Alcalde, M.
Título: Modified unspecific peroxygenase and uses thereof. Número de solicitud: EP17382151.3. País de solicitud: ESPAÑA. Entidad titular: REPSOL S.A.
Fecha de presentación de la solicitud: 24/05/2017.

2. Inventores (p.o. de firma): Alcalde, M. (con porcentaje mayoritario de participación), Molina-Espeja, P., Garcia-Ruiz, E., Gonzalez-Perez, D., Ullrich, R., Hofrichter, M.
Título: “Unspecific peroxygenase with high monooxygenase activity”. Número de solicitud: P201430595.País de solicitud: ESPAÑA. Entidad titular: C.S.I.C.
Fecha de presentación de la solicitud: 22/04/2014.

3. Inventores (p.o. de firma): García-Ruiz, E., Martínez, M.J., Dueñas, J., Martínez, A.T. and Alcalde, M. (con porcentaje mayoritario de participación).
Título: “High redox potential peroxidases designed by directed evolution”. País de solicitud: ESPAÑA. Número de solicitud: P200930157. Entidad titular: C.S.I.C.
Fecha de presentación de la solicitud: 12/05/2009.
Extensión PCT: PCT/ES2010/070316. International Ref: WO/2010/130862.
Fecha de presentación de la solicitud PCT: 11/05/2010.
Empresa/s que la están explotando: Material Transfer Agreement con la empresa Novozymes.