Authors: Garcia-Molina, G; Natale, P; Valenzuela, L; Alvarez-Malmagro, J; Gutierrez-Sanchez, C; Iglesias-Juez, A; Lopez-Montero, I; Velez, M; Pita, M; De Lacey, AL

Bioelectrochemistry. vol: 133. page: 1567-5394.
Date: JUN. 2020.
Doi: 10.1016/j.bioelechem.2020.107490.

Adenosine triphosphate (ATP) is a key molecule as energy vector for living organisms, therefore its detection reveals the presence of microbial colonies. Environments where the existence of microbial pathogens suppose a health hazard can benefit from real time monitoring of such molecule. We report a potentiometric biosensor based on ATP-synthase from Escherichia coli reconstituted in a floating phospholipid bilayer over gold electrodes modified with a 4-aminothiophenol self-assembled monolayer. The use of a pH-dependent redox probe on the electrode surface allows a simple, specific and reliable on site determination of ATP concentration from 1 mu M to 1 mM. The broad range ATP biosensor can offer an alternative way of measuring in a few minutes the presence of microbial contamination. (C) 2020 Elsevier B.V. All rights reserved..