The β-galactosidase immobilization protocol determines its performance as catalysts in the kinetically controlled synthesis of lactulose

Authors: Neto, CACG; Silva, NCGE; Costa, TD; de Albuquerque, TL; Goncalves, LRB; Fernandez-Lafuente, R; Rocha, MVP

Article.
Int. J. Biol. Macromol.. vol: 176. page: 0141-8130.
Date: APR 15. 2021.
Doi: 10.1016/j.ijbiomac.2021.02.078.

Abstract:
In this paper, 3 different biocatalysts of beta-galactosidase from Kluyveromyces lactis have been prepared by immobilization in chitosan activated with glutaraldehyde (Chi_Glu_Gal), glyoxyl agarose (Aga_Gly_Gal) and [read more]

Ficin: A protease extract with relevance in biotechnology and biocatalysis

Authors: Morellon-Sterling, R; El-Siar, H; Tavano, OL; Berenguer-Murcia, A; Fernandez-Lafuente, R Review. Int. J. Biol. Macromol.. vol: 162. page: 0141-8130. Date: nov-01. 2020. Doi: 10.1016/j.ijbiomac.2020.06.144. Abstract: Due to the problems raised by the use of animal or microbial recombinant proteases, the use of proteases from vegetable origin is becoming increasingly popular.. Among them, sulfidryl proteases have [read more]

Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering

Authors: Arana-Pena, S; Rios, NS; Mendez-Sanchez, C; Lokha, Y; Carballares, D; Goncalves, LRB; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 145. page: 0141-8130.
Date: feb-15. 2020.
Doi: 10.1016/j.ijbiomac.2019.10.087.

Abstract:
This paper shows the step by step coimmobilization of up to five different enzymes following two different orders in the coimmobilization to alter the effect of substrate diffusion limitations. The enzymes were [read more]

Use of glyoxyl-agarose immobilized ficin extract in milk coagulation: Unexpected importance of the ficin loading on the biocatalysts

Authors: Siar, E; Morellon-Sterling, R; Zidoune, MN; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 144. page: 0141-8130.
Date: feb-01. 2020.
Doi: 10.1016/j.ijbiomac.2019.12.140.

Abstract:
A protein extract obtained from fig tree (Ficus carica) latex containing ficin activity was immobilized on glyoxyl agarose. Different biocatalyst loadings were used (3, 10, 30 and 85 mg/g). When casein was used as substrate, the expressed activities [read more]

Optimized immobilization of polygalacturonase from Aspergillus niger following different protocols: Improved stability and activity under drastic conditions

Authors: Dal Magro, L; Kornecki, JF; Klein, MP; Rodrigues, RC; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 138. page: 0141-8130.
Date: oct-01. 2019.
Doi: 10.1016/j.ijbiomac.2019.07.092.

Abstract:
Polygalacturonase (PG) from Aspergillus niger was immobilized using glyoxyl, vinylsulfone or glutaraldehyde-activated supports. The use of supports pre-activated with glutaraldehyde presented the best results. The immobilization of PG on glutaraldehyde-supports was studied under different conditions: [read more]

Optimized immobilization of polygalacturonase from Aspergillus niger following different protocols: Improved stability and activity under drastic conditions

Authors: Dal Magro, L; Kornecki, JF; Klein, MP; Rodrigues, RC; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 138. page: 0141-8130.
Date: oct-01. 2019.
Doi: 10.1016/j.ijbiomac.2019.07.092.

Abstract:
Polygalacturonase (PG) from Aspergillus niger was immobilized using glyoxyl, vinylsulfone or glutaraldehyde-activated supports. The use of supports pre-activated with glutaraldehyde presented the best results. The immobilization of PG on glutaraldehyde-supports was studied under different conditions: [read more]

Amination of ficin extract to improve its immobilization on glyoxyl-agarose: Improved stability and activity versus casein

Authors: Siar, E; Morellon-Sterling, R; Zidoune, MN; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 133. page: 0141-8130.
Date: jul-15. 2019.
Doi: 10.1016/j.ijbiomac.2019.04.123.

Abstract:
Ficin extract has been aminated using ethylenediamine and carbodiimide to transform all exposed carboxylic groups into amino groups, retaining around 80% of activity versus benzoyl-D,L-argininep-nitroanilide hydrochloride (BANA) and 90% versus casein. This aminated enzyme was then immobilized on [read more]

New applications of glyoxyl-octyl agarose in lipases co-immobilization: Strategies to reuse the most stable lipase

Authors: Arana-Pena, S; Mendez-Sanchez, C; Rios, NS; Ortiz, C; Goncalves, LRB; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 131. page: 0141-8130.
Date: jun-15. 2019.
Doi: 10.1016/j.ijbiomac.2019.03.163.

Abstract:
Lipase B from Candida antarctica (CALB), lipase from Rhizomucor miehei (RML) and phospholipase Lecitase Ultra (LEU) were immobilized via interfacial activation and their stabilities were compared. Immobilized CALB was much more stable than immobilized [read more]

Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering

Authors: Arana-Pena, S; Rios, NS; Mendez-Sanchez, C; Lokha, Y; Carballares, D; Goncalves, LRB; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 145. page: 0141-8130.
Date: feb-15. 2020.
Doi: 10.1016/j.ijbiomac.2019.10.087.

Abstract:
This paper shows the step by step coimmobilization of up to five different enzymes following two different orders in the coimmobilization to alter the effect of substrate diffusion limitations. The enzymes were [read more]

Use of glyoxyl-agarose immobilized ficin extract in milk coagulation: Unexpected importance of the ficin loading on the biocatalysts

Authors: Siar, E; Morellon-Sterling, R; Zidoune, MN; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 144. page: 0141-8130.
Date: feb-01. 2020.
Doi: 10.1016/j.ijbiomac.2019.12.140.

Abstract:
A protein extract obtained from fig tree (Ficus carica) latex containing ficin activity was immobilized on glyoxyl agarose. Different biocatalyst loadings were used (3, 10, 30 and 85 mg/g). When casein was used as substrate, the expressed activities [read more]

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