Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering

Authors: de Albuquerque, TL; Gomes, SDL; D’Almeida, AP; Fernandez-Lafuente, R; Goncalves, LRB; Rocha, MVP

Article.
Process Biochem.. vol: 73. page: 1359-5113.
Date: OCT. 2018.
Doi: 10.1016/j.procbio.2018.08.010.

Abstract:
The aim of this work was to establish optimal conditions for the enzymatic production of lactulose by beta-galactosidase from Kluyveromyces lactis using cheese whey as feedstock. Initially, the production of this disaccharide was evaluated using [leer más]

Authors: Siar, E; Arana-Pena, S; Barbosa, O; Zidoune, MN; Fernandez-Lafuente, R

Article.
Process Biochem.. vol: 73. page: 1359-5113.
Date: OCT. 2018.
Doi: 10.1016/j.procbio.2018.07.013.

Abstract:
Ficin extract immobilized on glyoxyl-agarose was chemically modified using carbodiimide and ethylenediamine (full or partial amination of the carboxylic groups of the enzyme) or glutaraldehyde (modifying primary amino groups) under different conditions. Aminated enzyme altered its activity versus [leer más]

Authors: Santos-Moriano, P; Kidibule, PE; Alleyne, E; Ballesteros, AO; Heras, A; Fernandez-Lobato, M; Plou, FJ

Article.
Process Biochem.. vol: 73. page: 1359-5113.
Date: OCT. 2018.
Doi: 10.1016/j.procbio.2018.07.017.

Abstract:
Starting from a commercial preparation of Bacillus thuringiensis var. aizawai, widely employed as biopesticide in agriculture, we obtained a chitosanolytic activity by washing the cells with concentrated sodium acetate. The optimum pH and temperature [leer más]

Authors: de Oliveira, SM; Moreno-Perez, S; Terrasan, CRF; Romero-Fernandez, M; Vieira, MF; Guisan, JM; Rocha-Martin, J

Article.
Process Biochem.. vol: 64. page: 1359-5113.
Date: JAN. 2018.
Doi: 10.1016/j.procbio.2017.09.018.

Abstract:
The production of xylooligosaccharides (XOS) was evaluated using immobilized and stabilized biocatalysts of a commercial enzymatic cocktail, Bioxilanase L PLUS (BIO), which is based on the xylanolytic enzymes produced by Trichoderma reesei. BIO [leer más]

Authors: Zaak, H; Sassi, M; Fernandez-Lafuente, R

Article.
Process Biochem.. vol: 64. page: 1359-5113.
Date: JAN. 2018.
Doi: 10.1016/j.procbio.2017.09.020.

Abstract:
The paper shows the preparation of a new heterofunctional agarose support: amino-vinylsulfone. This has been employed to immobilize the interesting enzyme beta-galactosidase from Aspergillus oryzae. The enzyme cannot be immobilized on just vinylsulfone activated support a pH values ranging from 5.0 to [leer más]