The bone-degrading enzyme machinery: From multi-component understanding to the treatment of residues from the meat industry

Destacados, Publications 2021

The bone-degrading enzyme machinery: From multi-component understanding to the treatment of residues from the meat industry

Autores: Fernandez-Lopez, L; Sanchez-Carrillo, S; Garcia-Moyano, A; Borchert, E; Almendral, D; Alonso, S; Cea-Rama, I; Miguez, N; Larsen, O; Werner, J; Makarova, KS; Plou, FJ; Dahlgren, TG; Sanz-Aparicio, J; Hentschel, U; Bjerga, GEK; Ferrer, M

Artículo.
Comp. Struct. Biotechnol. J… vol: 19. page: 2001-0370.
Fecha: . 2021.
Doi: 10.1016/j.csbj.2021.11.027.

Resumen:
Many microorganisms feed on the tissue and recalcitrant bone materials from dead [read more]

Arturo Pérez2022-01-09T19:39:33+01:00Categories: Destacados, Publications 2021|Tags: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology|

Structure and evolutionary trace-assisted screening of a residue swapping the substrate ambiguity and chiral specificity in an esterase

Destacados, Publications 2021

Structure and evolutionary trace-assisted screening of a residue swapping the substrate ambiguity and chiral specificity in an esterase

Authors: Cea-Rama, I; Coscolin, C; Katsonis, P; Bargiela, R; Golyshin, PN; Lichtarge, O; Ferrer, M; Sanz-Aparicio, J

Article.
Comp. Struct. Biotechnol. J… vol: 19. page: 2001-0370.
Date: . 2021.
Doi: 10.1016/j.csbj.2021.04.041.

Abstract:
Our understanding of enzymes with high substrate ambiguity remains limited because their large active sites allow substrate docking freedom to an extent that seems incompatible with stereospecificity. One possibility is [read more]

Arturo Pérez2021-10-01T13:37:37+02:00Categories: Destacados, Publications 2021|Tags: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology|

Effect of Tris Buffer in the Intensity of the Multipoint Covalent Immobilization of Enzymes in Glyoxyl-Agarose Beads

Authors: Braham, SA; Morellon-Sterling, R; de Andrades, D; Rodrigues, RC; Siar, EH; Aksas, A; Pedroche, J; Millan, MD; Fernandez-Lafuente, R

Article; Early Access.
Appl. Biochem. Biotechnol.. vol: . page: 0273-2289.
Date: . .
Doi: 10.1007/s12010-021-03570-4.

Abstract:
Tris is an extensively used buffer that presents a primary amine group on its structure. In the present work trypsin, chymotrypsin and penicillin G acylase (PGA) [read more]

Arturo Pérez2021-07-01T18:02:36+02:00Categories: Publications 2021|Tags: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology|

Two additives to improve stability of immobilized lipase

Authors: Blanco, RM; Roldan, I

Article.
Biocatal. Biotransform.. vol: 36. page: 1024-2422.
Date: . 2018.
Doi: 10.1080/10242422.2017.1314466.

Abstract:
The presence of two different additives during non-covalent immobilization of lipase was studied. Lipase was immobilized via hydrophobic interactions on an amorphous silica with large pore size bearing octyl groups on the surface. Polyethyleneglycol (PEG) with different molecular weights (MW: 1500, 3000 and 10,000) [read more]

Arturo Pérez2018-05-29T20:18:00+02:00Categories: Publicaciones 2018|Tags: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology|

Optimization of the coating of octyl-CALB with ionic polymers to improve stability and decrease enzyme leakage

Authors: Fernandez-Lopez, L; Virgen-Ortiz, JJ; Pedrero, SG; Lopez-Carrobles, N; Gorines, BC; Otero, C; Fernandez-Lafuente, R

Article.
Biocatal. Biotransform.. vol: 36. page: 1024-2422.
Date: . 2018.
Doi: 10.1080/10242422.2016.1278212.

Abstract:
Lipase B from Candida antarctica (CALB) immobilized on octyl-agarose (OC) was submitted to coating with polyethylenimine (PEI) and dextran sulfate (DS). Using lowly loaded enzyme preparations, the properties of OC-CALB preparations hardly improved, suggesting [read more]

Arturo Pérez2018-05-29T20:18:00+02:00Categories: Publicaciones 2018|Tags: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology|

Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology

The bone-degrading enzyme machinery: From multi-component understanding to the treatment of residues from the meat industry

The bone-degrading enzyme machinery: From multi-component understanding to the treatment of residues from the meat industry

Structure and evolutionary trace-assisted screening of a residue swapping the substrate ambiguity and chiral specificity in an esterase

Structure and evolutionary trace-assisted screening of a residue swapping the substrate ambiguity and chiral specificity in an esterase

Effect of Tris Buffer in the Intensity of the Multipoint Covalent Immobilization of Enzymes in Glyoxyl-Agarose Beads

Two additives to improve stability of immobilized lipase

Optimization of the coating of octyl-CALB with ionic polymers to improve stability and decrease enzyme leakage

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