Geranyl Functionalized Materials for Site-Specific Co-Immobilization of Proteins

Authors: Brabcova, J; Andreu, A; Aguilera, D; Cabrera, Z; de las Rivas, B; Munoz, R; Palomo, JM

Article.
Molecules. vol: 26. page: .
Date: MAY. 2021.
Doi: 10.3390/molecules26103028.

Abstract:
Different materials containing carboxylic groups have been functionalized with geranyl-amine molecules by using an EDC/NHS strategy. Chemical modification of the support was confirmed by XRD, UV-spectrophotometer, and FT-IR. This geranyl-functionalized material was successfully [read more]

The β-galactosidase immobilization protocol determines its performance as catalysts in the kinetically controlled synthesis of lactulose

Authors: Neto, CACG; Silva, NCGE; Costa, TD; de Albuquerque, TL; Goncalves, LRB; Fernandez-Lafuente, R; Rocha, MVP

Article.
Int. J. Biol. Macromol.. vol: 176. page: 0141-8130.
Date: APR 15. 2021.
Doi: 10.1016/j.ijbiomac.2021.02.078.

Abstract:
In this paper, 3 different biocatalysts of beta-galactosidase from Kluyveromyces lactis have been prepared by immobilization in chitosan activated with glutaraldehyde (Chi_Glu_Gal), glyoxyl agarose (Aga_Gly_Gal) and [read more]

Comparing Ligninolytic Capabilities of Bacterial and Fungal Dye-Decolorizing Peroxidases and Class-II Peroxidase-Catalases

Authors: Linde, D; Ayuso-Fernandez, I; Laloux, M; Aguiar-Cervera, JE; de Lacey, AL; Ruiz-Duenas, FJ; Martinez, AT

Article.
Int. J. Mol. Sci.. vol: 22. page: .
Date: MAR. 2021.
Doi: 10.3390/ijms22052629.

Abstract:
We aim to clarify the ligninolytic capabilities of dye-decolorizing peroxidases (DyPs) from bacteria and fungi, compared to fungal lignin peroxidase (LiP) and versatile peroxidase (VP). With this purpose, DyPs from Amycolatopsis [read more]

Controlling Particle Morphology and Pore Size in the Synthesis of Ordered Mesoporous Materials

Authors: Awoke, Y; Chebude, Y; Diaz, I

Article.
Molecules. vol: 25. page: .
Date: NOV. 2020.
Doi: 10.3390/molecules25214909.

Abstract:
Ordered mesoporous materials have attracted considerable attention due to their potential applications in catalysis, adsorption, and separation technologies, as well as biomedical applications. In the present manuscript, we aim at a rational design to obtain the desired surface functionality (Ti and/or hydrophobic groups) [read more]

Ficin: A protease extract with relevance in biotechnology and biocatalysis

Authors: Morellon-Sterling, R; El-Siar, H; Tavano, OL; Berenguer-Murcia, A; Fernandez-Lafuente, R Review. Int. J. Biol. Macromol.. vol: 162. page: 0141-8130. Date: nov-01. 2020. Doi: 10.1016/j.ijbiomac.2020.06.144. Abstract: Due to the problems raised by the use of animal or microbial recombinant proteases, the use of proteases from vegetable origin is becoming increasingly popular.. Among them, sulfidryl proteases have [read more]

Sunlight-Operated TiO2-Based Photocatalysts

Authors: Barba-Nieto, I; Caudillo-Flores, U; Fernandez-Garcia, M; Kubacka, A Review. Molecules. vol: 25. page: . Date: SEP. 2020. Doi: 10.3390/molecules25174008. Abstract: Photo-catalysis is a research field with broad applications in terms of potential technological applications related to energy production and managing, environmental protection, and chemical synthesis fields. A global goal, common to all of these fields, [read more]

Natural Mordenite from Spain as Pozzolana

Authors: Presa, L; Costafreda, JL; Martin, DA; Diaz, I

Article.
Molecules. vol: 25. page: .
Date: mar-01. 2020.
Doi: 10.3390/molecules25051220.

Abstract:
This work deals with anomalous concentrations of natural mordenite in the southeast of Spain. The X-ray diffraction (XRD) and scanning electron microscopy (SEM) studies evidenced that the samples contain mainly monomineral zeolitic phase of mordenite (70% to 74%), usually accompanied by [read more]

Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering

Authors: Arana-Pena, S; Rios, NS; Mendez-Sanchez, C; Lokha, Y; Carballares, D; Goncalves, LRB; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 145. page: 0141-8130.
Date: feb-15. 2020.
Doi: 10.1016/j.ijbiomac.2019.10.087.

Abstract:
This paper shows the step by step coimmobilization of up to five different enzymes following two different orders in the coimmobilization to alter the effect of substrate diffusion limitations. The enzymes were [read more]

Use of glyoxyl-agarose immobilized ficin extract in milk coagulation: Unexpected importance of the ficin loading on the biocatalysts

Authors: Siar, E; Morellon-Sterling, R; Zidoune, MN; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 144. page: 0141-8130.
Date: feb-01. 2020.
Doi: 10.1016/j.ijbiomac.2019.12.140.

Abstract:
A protein extract obtained from fig tree (Ficus carica) latex containing ficin activity was immobilized on glyoxyl agarose. Different biocatalyst loadings were used (3, 10, 30 and 85 mg/g). When casein was used as substrate, the expressed activities [read more]

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