Biochemistry & Molecular Biology; Chemistry

Ficin: A protease extract with relevance in biotechnology and biocatalysis

Authors: Morellon-Sterling, R; El-Siar, H; Tavano, OL; Berenguer-Murcia, A; Fernandez-Lafuente, R Review. Int. J. Biol. Macromol.. vol: 162. page: 0141-8130. Date: nov-01. 2020. Doi: 10.1016/j.ijbiomac.2020.06.144. Abstract: Due to the problems raised by the use of animal or microbial recombinant proteases, the use of proteases from vegetable origin is becoming increasingly popular.. Among them, sulfidryl proteases have [leer más]

Sunlight-Operated TiO2-Based Photocatalysts

Authors: Barba-Nieto, I; Caudillo-Flores, U; Fernandez-Garcia, M; Kubacka, A Review. Molecules. vol: 25. page: . Date: SEP. 2020. Doi: 10.3390/molecules25174008. Abstract: Photo-catalysis is a research field with broad applications in terms of potential technological applications related to energy production and managing, environmental protection, and chemical synthesis fields. A global goal, common to all of these fields, [leer más]

Natural Mordenite from Spain as Pozzolana

Authors: Presa, L; Costafreda, JL; Martin, DA; Diaz, I

Article.
Molecules. vol: 25. page: .
Date: mar-01. 2020.
Doi: 10.3390/molecules25051220.

Abstract:
This work deals with anomalous concentrations of natural mordenite in the southeast of Spain. The X-ray diffraction (XRD) and scanning electron microscopy (SEM) studies evidenced that the samples contain mainly monomineral zeolitic phase of mordenite (70% to 74%), usually accompanied by [leer más]

Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering

Authors: Arana-Pena, S; Rios, NS; Mendez-Sanchez, C; Lokha, Y; Carballares, D; Goncalves, LRB; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 145. page: 0141-8130.
Date: feb-15. 2020.
Doi: 10.1016/j.ijbiomac.2019.10.087.

Abstract:
This paper shows the step by step coimmobilization of up to five different enzymes following two different orders in the coimmobilization to alter the effect of substrate diffusion limitations. The enzymes were [leer más]

Use of glyoxyl-agarose immobilized ficin extract in milk coagulation: Unexpected importance of the ficin loading on the biocatalysts

Authors: Siar, E; Morellon-Sterling, R; Zidoune, MN; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 144. page: 0141-8130.
Date: feb-01. 2020.
Doi: 10.1016/j.ijbiomac.2019.12.140.

Abstract:
A protein extract obtained from fig tree (Ficus carica) latex containing ficin activity was immobilized on glyoxyl agarose. Different biocatalyst loadings were used (3, 10, 30 and 85 mg/g). When casein was used as substrate, the expressed activities [leer más]

Optimized immobilization of polygalacturonase from Aspergillus niger following different protocols: Improved stability and activity under drastic conditions

Authors: Dal Magro, L; Kornecki, JF; Klein, MP; Rodrigues, RC; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 138. page: 0141-8130.
Date: oct-01. 2019.
Doi: 10.1016/j.ijbiomac.2019.07.092.

Abstract:
Polygalacturonase (PG) from Aspergillus niger was immobilized using glyoxyl, vinylsulfone or glutaraldehyde-activated supports. The use of supports pre-activated with glutaraldehyde presented the best results. The immobilization of PG on glutaraldehyde-supports was studied under different conditions: [leer más]

Optimized immobilization of polygalacturonase from Aspergillus niger following different protocols: Improved stability and activity under drastic conditions

Authors: Dal Magro, L; Kornecki, JF; Klein, MP; Rodrigues, RC; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 138. page: 0141-8130.
Date: oct-01. 2019.
Doi: 10.1016/j.ijbiomac.2019.07.092.

Abstract:
Polygalacturonase (PG) from Aspergillus niger was immobilized using glyoxyl, vinylsulfone or glutaraldehyde-activated supports. The use of supports pre-activated with glutaraldehyde presented the best results. The immobilization of PG on glutaraldehyde-supports was studied under different conditions: [leer más]

Immobilization of Lipase A from Candida antarctica onto Chitosan-Coated Magnetic Nanoparticles

Authors: Monteiro, RRC; Lima, PJM; Pinheiro, BB; Freire, TM; Dutra, LMU; Fechine, PBA; Goncalves, LRB; de Souza, MCM; dos Santos, JCS; Fernandez-Lafuente, R

Article.
Int. J. Mol. Sci.. vol: 20. page: 1661-6596.
Date: AUG 2. 2019.
Doi: 10.3390/ijms20164018.

Abstract:
In this communication, lipase A from Candida antarctica (CALA) was immobilized by covalent bonding on magnetic nanoparticles coated with chitosan and activated with [leer más]

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