Recent developments in the use of peroxygenases – Exploring their high potential in selective oxyfunctionalisations

Autores: Hobisch, M; Holtmann, D; de Santos, PG; Alcalde, M; Hollmann, F; Kara, S

Review.
Biotechnol. Adv.. vol: 51. page: 0734-9750.
Fecha: nov-01. 2021.
Doi: 10.1016/j.biotechadv.2020.107615.

Resumen:
Peroxygenases are an emerging new class of enzymes allowing selective oxyfunctionalisation reactions in a cofactor-independent way different from well-known P450 monooxygenases. Herein, we focused on recent de-velopments from organic synthesis, molecular biotechnology and reaction engineering [read more]

Enzyme co-immobilization: Always the biocatalyst designers’ choice … or not? …

Autores: Arana-Pena, S; Carballares, D; Morellon-Sterlling, R; Berenguer-Murcia, A; Alcantara, AR; Rodrigues, RC; Fernandez-Lafuente, R

Review.
Biotechnol. Adv.. vol: 51. page: 0734-9750.
Fecha: nov-01. 2021.
Doi: 10.1016/j.biotechadv.2020.107584.

Resumen:
The increasing relevance of cascade reactions in biocatalysis has sparked a great interest for enzyme coimmobilization. Enzyme co-immobilization allows access to some kinetic advantages that in some instances are necessary to get the desired [read more]

Effect of amine length in the interference of the multipoint covalent immobilization of enzymes on glyoxyl agarose beads

Authors: Morellon-Sterling, R; Siar, E; Braham, SA; de Andrades, D; Pedroche, J; Millan, MD; Fernandez-Lafuente, R

Article.
J. Biotechnol.. vol: 329. page: 0168-1656.
Date: mar-10. 2021.
Doi: 10.1016/j.jbiotec.2021.02.005.

Abstract:
Trypsin, chymotrypsin, penicillin G acylase and ficin extract have been stabilized by immobilization on glyoxyl agarose, adding different aliphatic compounds bearing a primary amine group during the immobilization: ethyl amine, butyl amine, hexyl [read more]

Effect of amine length in the interference of the multipoint covalent immobilization of enzymes on glyoxyl agarose beads

Authors: Morellon-Sterling, R; Siar, E; Braham, SA; de Andrades, D; Pedroche, J; Millan, MD; Fernandez-Lafuente, R

Article.
J. Biotechnol.. vol: 329. page: 0168-1656.
Date: 01-mar. 2021.
Doi: 10.1016/j.jbiotec.2021.02.005.

Abstract:
Trypsin, chymotrypsin, penicillin G acylase and ficin extract have been stabilized by immobilization on glyoxyl agarose, adding different aliphatic compounds bearing a primary amine group during the immobilization: ethyl [read more]

Magnetic micro-macro biocatalysts applied to industrial bioprocesses

Autores: Del Arco, J; Alcantara, AR; Fernandez-Lafuente, R; Fernandez-Lucas, J

Artículo.
Bioresour. Technol. vol: 322. página: 1873-2976
Fecha: feb. 2021.
Doi: 10.1016/j.biortech.2020.124547.

Resumen:
The use of magnetic biocatalysts is highly beneficial in bioprocesses technology, as it allows their easy recovering and enhances biocatalyst lifetime. Thus, it simplifies operational processing and increases efficiency, leading to more cost-effective processes. The [read more]

Molecular characterization and heterologous expression of two alpha-glucosidases from Metschnikowia spp, both producers of honey sugars

Authors: Garcia-Gonzalez, M; Minguet-Lobato, M; Plou, FJ; Fernandez-Lobato, M

Article.
Microb. Cell. Fact.. vol: 19. page: .
Date: jul-11. 2020.
Doi: 10.1186/s12934-020-01397-y.

Abstract:
Background: alpha-Glucosidases are widely distributed enzymes with a varied substrate specificity that are traditionally used in biotechnological industries based on oligo- and polysaccharides as starting materials. According to amino acid sequence homology, alpha-glucosidases are included into two major families, [read more]

Modulating the properties of the lipase from Thermomyces lanuginosus immobilized on octyl agarose beads by altering the immobilization conditions

Authors: Lokha, Y; Arana-Pena, S; Rios, NS; Mendez-Sanchez, C; Goncalves, LRB; Lopez-Gallego, F; Fernandez-Lafuente, R

Article.
Enzyme Microb. Technol.. vol: 133. page: 0141-0229.
Date: FEB. 2020.
Doi: 10.1016/j.enzmictec.2019.109461.

Abstract:
The lipase from Thermomyces lanuginosus (TLL) has been immobilized on octyl-agarose beads via interfacial activation under 16 different conditions (changing the immobilization pH, the ionic strength, the presence of additives like calcium, phosphate [read more]

Prolongation of secondary drying step of phospholipid lyophilization greatly improves acidolysis reactions catalyzed by immobilized lecitase ultra

Authors: Verdasco-Martin, CM; Corchado-Lopo, C; Fernandez-Lafuente, R; Otero, C

Article.
Enzyme Microb. Technol.. vol: 132. page: 0141-0229.
Date: JAN. 2020.
Doi: 10.1016/j.enzmictec.2019.109388.

Abstract:
Conjugated linolenic acid (CLA) has important health effects, and their phospholipids forms (PL) are advantageous vehicles of this bioactive agent. Acidolysis of soybean phosphatidylcholine (PC) with conjugated linolenic acid (CLA) catalyzed by Lecitase (R) Ultra immobilized on Duolite A658 [read more]

Pectin lyase immobilization using the glutaraldehyde chemistry increases the enzyme operation range

Authors: Dal Magro, L; Kornecki, JF; Klein, MP; Rodrigues, RC; Fernandez-Lafuente, R

Article.
Enzyme Microb. Technol.. vol: 132. page: 0141-0229.
Date: JAN. 2020.
Doi: 10.1016/j.enzmictec.2019.109397.

Abstract:
Pectin lyase (from Rohapect 10 L) was immobilized on glutaraldehyde supports at low ionic strength at pH 5, 6.5 or 8 and later incubated at pH 8 for 48 h. The activity recovery of the biocatalysts [read more]

Modulating the properties of the lipase from Thermomyces lanuginosus immobilized on octyl agarose beads by altering the immobilization conditions

Authors: Lokha, Y; Arana-Pena, S; Rios, NS; Mendez-Sanchez, C; Goncalves, LRB; Lopez-Gallego, F; Fernandez-Lafuente, R

Article.
Enzyme Microb. Technol.. vol: 133. page: 0141-0229.
Date: FEB. 2020.
Doi: 10.1016/j.enzmictec.2019.109461.

Abstract:
The lipase from Thermomyces lanuginosus (TLL) has been immobilized on octyl-agarose beads via interfacial activation under 16 different conditions (changing the immobilization pH, the ionic strength, the presence of additives like calcium, phosphate [read more]

Calendar

noviembre 2021
L M X J V S D
1234567
891011121314
15161718192021
22232425262728
2930  
Go to Top