Biotechnology & Applied Microbiology; Food Science & Technology

Stability/activity features of the main enzyme components of rohapect 10L

Authors: Dal Magro, L; Kornecki, JF; Klein, MP; Rodrigues, RC; Fernandez-Lafuente, R

Article.
Biotechnol. Prog.. vol: 35. page: 8756-7938.
Date: NOV-DEC. 2019.
Doi: 10.1002/btpr.2877.

Abstract:
Rohapect 10L is an enzyme cocktail commercialized for juice clarification. Here, we characterized the activity and stability of five enzymatic activities present in this cocktail: total pectinase (PE), polygalacturonase (PG), pectin lyase (PL), pectin methyl esterase (PME), [read more]

Kinetic Resolution of Drug Intermediates Catalyzed by Lipase B from Candida Antarctica Immobilized on Immobead-350

Authors: Pinheiro, MP; Rios, NS; Fonseca, TD; Bezerra, FD; Rodriguez-Castellon, E; Fernandez-Lafuente, R; de Mattos, MC; dos Santos, JCS; Goncalves, LRB

Article.
Biotechnol. Prog.. vol: 34. page: 8756-7938.
Date: JUL-AUG. 2018.
Doi: 10.1002/btpr.2630.

Abstract:
Novozyme 435, which is a commercial immobilized lipase B from Candida antarctica (CALB), has been proven to be inadequate for the kinetic resolution of rac-indanyl acetate. As it [read more]

1,3-Regiospecific Ethanolysis of Soybean Oil Catalyzed by Crosslinked Porcine Pancreas Lipase Aggregates

Authors: Ramos, MD; Miranda, LP; Giordano, RLC; Fernandez-Lafuente, R; Kopp, W; Tardioli, PW

Article.
Biotechnol. Prog.. vol: 34. page: 8756-7938.
Date: JUL-AUG. 2018.
Doi: 10.1002/btpr.2636.

Abstract:
The preparation of crosslinked aggregates of pancreatic porcine lipase (PPL-CLEA) was systematically studied, evaluating the influence of three precipitants and two crosslinking agents, as well as the use of soy protein as an alternative feeder protein [read more]

Enzymatic Synthesis of Ethyl Esters from Waste Oil Using Mixtures of Lipases in a Plug-Flow Packed-Bed Continuous Reactor

Authors: Poppe, JK; Matte, CR; de Freitas, VO; Fernandez-Lafuente, R; Rodrigues, RC; Ayub, MAZ

Article.
Biotechnol. Prog.. vol: 34. page: 8756-7938.
Date: JUL-AUG. 2018.
Doi: 10.1002/btpr.2650.

Abstract:
This work describes the continuous synthesis of ethyl esters via enzymatic catalysis on a packed-bed continuous reactor, using mixtures of immobilized lipases (combi-lipases) of Candida antarctica (CALB), Thermomyces lanuginosus (TLL), and Rhizomucor miehei (RML). The [read more]

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