Pita Marcos
Telephone: 915119784
Tel. interno: 437495
Group: Bioelectrocatalysis
Office: 212
Lab: 213/307


I achieved my PhD at ICP-CSIC working from 2002 to 2006 on nanoparticle engineering for the biomolecule immobilization. During this stage I performed one secondment at Utrecht University on magnetic nanoparticles and two secondments at Lund University (Sweden), where I started my research on laccase bioelectrochemistry. The latter research line flourished at ICP since then, yielding the founding of future international collaborations that are currently still active. A significant milestone achieved during my predoctoral stage was the development and patenting of gold-covered magnetic nanoparticles, and their use as biosensing material together with peptide nucleic acids or redox enzymes.
In Jan 2007 I joined Clarkson University (NY, USA) as a Post-doctoral Research Assistant with Prof. Evgeny Katz. The research focused on bioelectrochemical systems and enzyme biofuel cells. I set up the laboratory, as both Prof. and me were newcomers, afterwards contributed in all the scientific aspects in the research group. During this stage I was the in-charge advisor of a group comprising 4-5 PhD candidates and 2-3 post-doctoral researchers. Year 2009 I obtained a Ramon y Cajal tenure-track type contract, which allowed my reincorporation to ICP-CSIC early 2010.
From 2010 to 2014 I developed my research at ICP-CSIC working on nanostructured materials for bioelectrochemical processes together with Prof. Victor Fernandez and Dr. Antonio L. De Lacey, joining European and National projects. I achieved my tenure in 2013 call (only 5 positions for all CSIC) and was incorporated in January 2015 Staff Researcher in Biocatalysis Department, ICP, where currently I am still working.

Research Interests:
•Biofunctionalized nanomaterials (metallic nanoparticles, magnetic nanoparticles, semiconductive quantum dots, nanorods, nanowires, graphene, nano-carbon derivatives) for bioelectronic and biomedical applications.
•Bionanoengineering, functional interfaces composed of biomaterials & nano-objects, supra-molecular hybrid systems with complex molecular/biomolecular architecture;
•Bioelectronics & optobioelectronics, switchable & tunable electrobiocatalytic and photobiocatalytic systems;
•Bionanotechnology for bioelectronic applications, electrical wiring of enzymes by nano-objects, amplification paths for bioanalytical systems, nanocircuitry generation based on biotemplates & biocatalysis;
•Biosensors based on integrated systems with complex molecular architecture, self-powered biosensors, switchable & tunable biosensors;
•Photobioelectrochemistry, transform light energy into reduced chemical compounds by means of biocatalysts coupled to electroactive surfaces;
•Biofuel cells engineering, biofuel cells based on integrated bioelectrocatalytic systems


106. Gabriel Garcia-Molina, Paolo Natale, Laura Valenzuela, Julia Alvarez-Malmagro, Cristina Gutierrez-Sanchez, Ana Iglesias-Juez, Ivan Lopez-Montero, Marisela Velez, Marcos Pita*, Antonio L. De Lacey*. “Potentiometric detection of ATP base don the transmembrane proton gradient generated by ATPase reconstituted on a gold electrode.” Bioelectrochemistry, 2020, in press.

105. Gabriel Garcia-Molina, Marcos Pita, Antonio L. De Lacey. “Novel Bioelectrocatalytic Strategies Base don Immobilized Redox Metalloenzymes on Tailored Electrodes”. Chapter 10, pages 207-229, in the book “Novel Catalyst Materials for Bioelectrochemical Systems: Fundamentals and Applications”. Ed. Singh et al. ACS Symposium Series, ACS: Washington DC, 2020.

104. Jose M. Abad, Marcos Pita, Victor M Fernandez. “Immobilization of Proteins on Gold Surfaces.” Chapter 13 pages 199-209 in the book “Immobilization of Enzymes and Cells: Methods and Protocols”, vol. 2100. Ed. J. M. Guisan et al. Humana Press, Springer Science, Hatfield, UK, 2020.

103. Julian Szczesny, Adrian Ruff, Ana R. Oliveira, Marcos Pita, Inês A. C. Pereira,
Antonio L. De Lacey, Wolfgang Schuhmann. “Electroenzymatic CO2 Fixation Using Redox Polymer/Enzyme-Modified Gas Diffusion Electrodes.” ACS Energy Letters 2020, 5, 321-327. DOI: 10.1021/acsenergylett.9b02436.

102. Sandra Alonso, Gerard Santiago, Isabel Cea-Rama, Laura Fernández-López, Cristina Coscolín, Jan Modregger, Anna K. Ressmann, Mónica Martínez-Martínez, Helena Marrero, Rafael Bargiela, Marcos Pita, Jose L. Gonzalez-Alfonso, Manon L. Briand, David Rojo, Coral Barbas, Francisco J Plou, Peter N. Golyshin, Patrick Shahgaldian, Julia Sanz-Aparicio, Víctor Guallar, Manuel Ferrer. “A Genetically Engineered Protein for Synergistic Chemo- and Bio-Catalysis” Nature Catalysis, 2019, in press. DOI: 10.1038/s41929-019-0394-4.

101. Carmen Jarne, Logan Paul, Jose C. Conesa, Sergey Shleev, Antonio L. De Lacey, Marcos Pita*. “Underpotential Photoelectrooxidation of Water by SnS2-Laccase Co-catalysts on Nanostructured Electrodes with Only Visible Light Irradiation”. ChemElectroChem 2019, 6, 2755-2761. DOI: 10.1002/celc.201900360.

100. Sónia Zacarias, Marisela Vélez, Marcos Pita, António L. De Lacey, Pedro M. Matias, Inês A. C. Pereira. “Purification and Characterization of the [NiFeSe] hydrogenase from Desulfovibrio vulgaris Hildenborough”. Methods in Enzymology 2018, Vol 613 Enzymes of Energy Technology, chapter 7, 169-202. Ed. Fraser Armstrong, Elsevier, Oxford, UK.

99. Isabel Fuentes, Tania García-Mendiola, Shinichi Sato, Marcos Pita, Hiroyuki Nakamura, Encarnación Lorenzo, Francesc Teixidor, Fernanda Marques, Clara Viñas. “Metallacarboranes on the road to anticancer therapies: cellular uptake, DNA interaction and in vivo evaluation of cobaltabisdicarbollide ([COSAN]-)”, Chemistry: an European Journal, 2018, 24 (65), 17239-17254. DOI:10.1002/chem.201803178.

98. Galina Pankratova, Dmitry Pankratov, Chiara Di Bari, Asier Goñi-Urtiaga, Miguel D. Toscano, Qijin Chi, Marcos Pita, Lo Gorton, Antonio L. De Lacey. “Three-Dimensional Graphene Matrix- Supported and Thylakoid Membrane-Based High-Performance Bioelectrochemical Solar Cell”. ACS Applied Energy Materials 2018, 1, 319-323. DOI 10.1021/acsaem.7b00249.

97. Sofiene Abdellaoui, Florika C. Macazo, Rong Cai, Antonio L. De Lacey, Marcos Pita, and Shelley D. Minteer. “Enzymatic Electrosynthesis of Alkanes via Bioelectrocatalytic Decarbonylation of Fatty Aldehydes”. Angewandte Chemie International Edition, 2018, 57, 2404-2408. DOI 10.1002/anie.201712890.

96. Marcos Pita, Cristina Gutierrez-Sanchez, Paolo Natale, Gabriel García-Molina, Ileana F. Marquez, Marta C. Marques, Sonia Zacarias, Ines A. C. Pereira, Ivan Lopez-Montero, Marisela Velez and Antonio L. De Lacey. “ATP Synthesis and Biosensing Coupled to the Electroenzymatic Activity of a Hydrogenase on an Electrode/Biomimetic Membrane Interface”. Proceedings 2017, 1, 766. DOI: 10.3390/proceedings1080766.

95. Chiara Di Bari, Nicolas Mano, Sergey Shleev, Marcos Pita, Antonio L. De Lacey. “Spectroelectrochemical studies of halides inhibition mechanism of multi copper oxidases using azide as an active infrared probe”. Journal of Biological Inorganic Chemistry, 2017, 22, 1179-1186. DOI: 10.1007/s00775-017-1494-8.

94. Cristina Tapia, Sergey Shleev, José Carlos Conesa, Antonio L. De Lacey, Marcos Pita. “Laccase- Catalyzed Bioelectrochemical Oxidation of Water Assisted with Visible Light”. ACS Catalysis, 2017, 7, 4881-4889. DOI: 10.1021/acscatal.7b01556.

93. Elena Gonzalez-Arribas, Tim Bobrowski, Chiara Di Bari, Kirill Sliozberg, Roland Ludwig, Miguel D Toscano, Antonio L De Lacey, Marcos Pita, Wolfgang Schuhmann, Sergey Shleev. “Transparent, Mediator- and Membrane-free Enzymatic Fuel Cell Based on Nanostructured Chemically Modified Indium Tin Oxide Electrodes”. Biosensors & Bioelectronics, 2017, 97, 46-52. DOI: 10.1016/j.bios.2017.05.040.

92. Marcos Pita. “Enzyme Biocomputing: Logic Gates and Logic Networks to Interface and Control Materials”. Chapter 20, Biocatalysis and Nanotechnology. Ed. Peter Grunwald. 2017 Pan Stanford Publishing Pte. Ltd. ISBN 978-981-4613-69-9 (Hard cover); 978-131-5196-60-2 (eBook).

91. Fernando Otero, Chiara di Bari, Antonio L. De Lacey, Marcos Pita. “Immobilization and Electrochemical Characterization of Coriolopsis Gallica High Redox Potential Laccase”. Chapter 5 Pages 101-110, Laccase: Applications, Investigations and Insights. Ed. Amanda Harris. 2017 Nova Science Publishers, Inc. ISBN: 978-1-53610-556-8.

90. Chiara di Bari, Marcos Pita, Antonio L. De Lacey. “Laccase-Based Biocathodes for Oxygen Electroreduction in Enzymatic Fuel Cells.” Chapter 4 Pages 75-100, Laccase: Applications, Investigations and Insights. Ed. Amanda Harris. 2017 Nova Science Publishers, Inc. ISBN: 978-1-53610-556-8.

89. Jana Lutze, Miguel A. Bañares, Marcos Pita, Andrea Haase, Andreas Luch, Andreas Taubert. “α- ((4-Cyanobenzoyl)oxy)-ω-methyl poly(ethylene glycol): a new stabilizer for silver nanoparticles”. Beilstein J Nanotechnoly, 2017, 8, 627-635. DOI: 10.3762/bjnano.8.67

88.Ross D. Milton, Rong Cai, Sofiene Abdellaoui, Dónal Leech, Antonio L. De Lacey, Marcos Pita, Shelley Minteer. “Bioelectrochemical Haber-Bosch Process: An Ammonia-Producing H2/N2 Fuel Cell.” Angewandte Chemie International Edition, 2017, 56, 2680-2683. DOI: 10.1002/anie.201612500.
Marked as Very Important Paper by Angewandte Chemie’s editors.

87.Till Siepenkoetter, Urszula Salaj-Kosla, Xinxin Xiao, Peter O Conghaile, Marcos Pita, Roland Ludwig, Edmond Magner. “Immobilisation of redox enzymes on nanoporous gold electrodes: applications in biofuel cells”. ChemPlusChem, 2017, 82, 553-560. DOI: 10.1002/cplu.201600455.

86. Cristina Tapia, Ross D. Milton, Galina Pankratova, Shelley Minteer, Hans-Erik Åkerlund, Dónal Leech, Antonio L. de Lacey, Marcos Pita, Lo Gorton. “Wiring of Photosystem I and Hydrogenase on an Electrode for Photoelectrochemical H2 Production using Redox Polymers for Relatively Positive Onset Potential” ChemElectroChem, 2017, 4 (1), 90-95. DOI: 10.1002/celc.201600506.

85. Cristina Tapia, Sean P. Berglund, Dennis Friedrich, Thomas Dittrich, Peter Bogdanoff, Yang Liu, Sergiu Levcenco, Thomas Unold, José Carlos Conesa, Antonio L. de Lacey, Marcos Pita*, Sebastian Fiechter. “Synthesis and Characterization of V-doped β-In2S3 Thin Films on FTO substrates”. Journal of Physical Chemistry C, 2016, 120, 28753-28761. DOI: 10.1021/acs.jpcc.6b09601.

84. Cristina Tapia, Sonia Zacarias, Inês A. C. Pereira, Jose C. Conesa, Marcos Pita, Antonio L. De Lacey. “In situ determination of photobioproduction of H2 by In2S3-[NiFeSe] Hydrogenase from Desulfovibrio vulgaris Hildenborough using only visible light”. ACS Catalysis, 2016, 6, 5691-5698. DOI: 10.1021/acscatal.6b01512.

83.Óscar Gutiérrez-Sanz, Paolo Natale, Ileana Márquez, Marta C. Marques, Sonia Zacarías, Marcos Pita, Inês A. C. Pereira, Iván López-Montero, Antonio L. De Lacey, Marisela Vélez. “H2 fueled ATP synthesis on an electrode: mimicking cellular respiration”. Angewandte Chemie International Edition, 2016, 55, 6216-6220. DOI: 10.1002/anie.201600752.
Marked as Hot Paper by editors.
Highlighted: Nature Nanotechnology. Alberto Mocatelly. “Fuel to Fuel” (05-May-2016) doi: 10.1038/nnano.2016.77

82. Chiara Di Bari, Asier Goñi-Urtiaga, Marcos Pita, Sergey Shleev, Miguel D. Toscano, Raquel Sainz, Antonio L. De Lacey. “Fabrication of high surface area graphene electrodes with high performance towards enzymatic oxygen reduction”. Electrochimica Acta, 2016, 191, 500-509.

81. Yun Liu, Heeralal Vignesh Babu, Jianquing Zhao, Asier Goñi-Urtiaga, Raquel Sainz, Rafael Ferritto, Marcos Pita, De-Yi Wang. “Effect of Cu-doped graphene on the flammability and termal properties of epoxy composites”. Composites Part B; Engineering, 2016, 89, 108-116. DOI: 10.1016/j.compositesb.2015.11.035.

80. Chiara di Bari, Sergey Shleev, Antonio L. De Lacey, Marcos Pita. “Laccase-Modified Gold Nanorods for Electrocatalytic Reduction of Oxygen” Bioelectrochemistry 2016, 107, 30-36. DOI: 10.1016/j.bioelechem.2015.10.003.

79. Evgeny Katz, Víctor M Fernandez, Marcos Pita. “Switchable Bioelectrocatalysis Controlled by pH Change”. Electroanalysis 2015, 27,02063-2073.

78. César Gómez-Anquela, Tania García-Mendiola, José M. Abad, Marcos Pita, Felix Pariente, Encarna Lorenzo. “Scaffold electrodes based on Thioctic acid-capped gold nanoparticles coordinated Alcohol Dehydrogenase and azure A films for high performance biosensor”. Bioelectrochemistry 2015, 106, 335-342.

77. Cristina Gutierrez-Sanchez, Sergey Shleev, Antonio L. De Lacey, Marcos Pita. “Third-generation Oxygen Amperometric Biosensor based on Trametes hirsuta Laccase Covalently Bound to Graphite Electrode”. Chemical Papers 2015, 69(1), 237-240. DOI: 10.2478/s11696-014-0595-x.

76. Oscar Gutierrez-Sanz, David Olea, Marcos Pita, Ana P. Batista, Alvaro Alonso, Manuela M. Pereira, Marisela Vélez, Antonio L. De Lacey. “Reconstitution of respiratory complex I on a biomimetic membrane supported on gold electrodes” Langmuir 2014, 30, 9007-9015. DOI: 10.1021/la501825r.

75. Magnus Falk, Miguel Alcalde, Phil Bartlett, Antonio L. De Lacey, Lo Gorton, Cristina Gutierrez-Sanchez, Raoudha Haddad, Dónal Leech, Roland Ludwig, Edmund Magner, Diana M. Mate, PeterÓ Conghaile, Roberto Ortiz, Marcos Pita, Sascha Pöller, Tautgirdas Ruzgas, Urszula Salaj-Kosla,Wolfgang Schuhmann, Fredrik Sebelius, Minling Shao, Leonard Stoica, Jonas Tilly, Miguel D.Toscano, Jeevanthi Vivekananthan, Emma Wright, Sergey Shleev. “Self- contained biodevices for carbohydrate and oxygen monitoring” PLoS ONE, 2014, 9, e109104.

74. Marcos Pita, Diana Mate Mate, David Gonzalez-Perez, Sergey Shleev, Víctor M. Fernandez, Miguel Alcalde, Antonio L. De Lacey. “Bioelectrochemical Oxidation of Water”. Journal of the American Chemical Society, 2014, 136, 5892-5895.

73. Marcos Pita, Cristina Gutierrez-Sanchez, Miguel D. Toscano, Sergey Shleev, Antonio L. De Lacey. “Oxygen Biosensor Based on Bilirubin Oxidase Immobilized on a Nanostructured Gold Electrode”. Bioelectrochemistry, 2013, 94, 69-74.

72. Cristina Gutiérrez-Sánchez, Marcos Pita, Miguel D. Toscano, Antonio L. De Lacey. “Bilirubin Oxidase-Based Nanobiocathode Working in Serum-Mimic Buffer for Implantable Biofuel Cell”. Electroanalysis, 2013, 25, 1359-1362.

71. Alemnew Geto, Marcos Pita, Antonio Lopez De Lacey, Merid Tessema, Shimelis Admassie. “Electrochemical determination of berberine at a multi-walled carbon nanotubes modified glassy carbon electrode” Sensors & Actuators: B. Chemical, 2013, 183, 96-101.

70. Diana M. Mate, David Gonzalez-Perez, Magnus Falk, Roman Kittl, Marcos Pita, Antonio L. De Lacey, Roland Ludwig, Sergey Shleev, Miguel Alcalde. “Blood tolerant laccase by directed evolution”. Chemistry and Biology, 2013, 20, 223-231.

69. Marcos Pita. “Switchable Biofuel Cells Controlled by Biomolecular Computing Systems”. International Journal of Unconventional Computing, 2012, 8, 391-417.

68. Cristina Gutierrez-Sanchez, Marcos Pita, Cristina Vaz-Domínguez, Sergey Shleev, Antonio L. De Lacey. “Gold nanoparticles as electronic bridges for laccase-based biocathodes” J. Am. Chem. Soc. 2012, 134, 17212-17220. DOI: 10.1021/ja307308j

67. Marcos Pita, Marina Privman, Evgeny Katz. “Biocatalytic Enzyme Networks Designed for Binary-Logic Control of Smart Electroactive Nanobiointerfaces”. Topics in Catalysis, 2012, 55(16-18), 1201-1216.

66. Cristina Gutiérrez-Sánchez, Wenzhi Jia, Yvonne Beyl, Marcos Pita, Wolfgang Schuhmann, Antonio L. De Lacey, Leonard Stoica. “Enhanced direct electron transfer between laccase and hierarchical carbon microfibers/carbon nanotubes composite electrodes. Comparison of three enzyme immobilization methods”. Electrochimica acta, 2012, 82, 218-223.

65. Cristina Vaz-Domínguez, Marcos Pita, Antonio L. de Lacey, Sergey Shleev, Angel Cuesta. “Combined ATR-SEIRAS and EC-STM Study of the Immobilization of Laccase on Chemically Modified Au Electrodes” Journal of Physical Chemistry C, 2012, 116, 16532-16540.

64. Marcos Pita, Jan Halámek, Soujanya Chinnapareddy, Donald J. White, Vladimir Gartstein, Evgeny Katz. “Permeability of Human Tooth Surfaces Studied In Vitro by Electrochemical Impedance Spectroscopy”. Electroanalysis, 2012, 24,1033-1038.

63. Sylvain Clot, Cristina Gutierrez-Sanchez, Sergey Shleev, Antonio L. De Lacey, Marcos Pita. “Laccase cathode approaches to physiological conditions by local pH acidification”. Electrochemistry Communications 2012, 18, 37-40.
62. Marcos Pita, Antonio López de Lacey. “Métodos Electroanalíticos.” Técnicas de Análisis y Caracterización de Materiales, 2nd Ed., C. Goberna (ed), Publicaciones del CSIC, 2012, Madrid, España.

61. Marcos Pita, Cristina Gutierrez-Sanchez, David Olea, Marisela Velez, Cristina García-Diego, Sergey Shleev, Víctor M. Fernandez, Antonio L. De Lacey. “High-Redox Potential Cathode Based on Laccase Covalently Attached to Gold Electrode” Journal of Physical Chemistry C, 2011, 115 (27), 13420-13428.

60. Marcos Pita, Vladimir Privman, Mary A. Arugula, Dmitriy Melnikov, Vera Bocharova, Evgeny Katz. “Towards Biochemical Filter with Sigmoidal Response to pH Changes: Buffered Biocatalytic Signal Transduction” Physical Chemistry Chemical Physics, 2011, 13, 4507-4513.

59. Evgeny Katz, Marcos Pita. “Biomedical Applications of Magnetic Particles (Fine Particles in Medicine and Pharmacy). Egon Matijevic (ed), Springer, 2011, New York.

58. Marcos Pita, Evgeny Katz. “Biocatalytic paradigms for unconventional information processing”. Journal of Computational and Theoretical Nanoscience, 2011, 8, 401-408.

57. Sergiy Minko, Evgeny Katz, Mikhail Motornov, Ihor Tokarev, Marcos Pita. “Materials with Built-in Logic” Journal of Computational and Theoretical Nanoscience, 2011, 8, 356-364.

56. Valber Pedrosa, Dmitriy Melnikov, Marcos Pita, Jan Halámek, Vladimir Privman, Aleksandr Simonian, Evgeny Katz. “Enzymatic Logic Gates with Noise-reducing Sigmoid Response” Journal of Unconventional Computing, 2010, 6 (6), 451-460.

55. Mary A. Arugula, Vera Bocharova, Jan Halámek, Marcos Pita, Evgeny Katz. “Enzyme-based multiplexer and demultiplexer”. Journal of Physical Chemistry B, 2010, 114 (15), 5222-5226.

54. Jan Halámek, Tsz Kin Tam, Guinevere Strack, Vera Bocharova, Marcos Pita, Evgeny Katz. “Self-powered biomolecular keypad lock security system based on a biofuel cell.” Chemical Communications 2010, (14), 2405-2407.
Article highlighted in Royal Society of Chemistry:

53. Vera Bocharova, Tsz Kin Tam, Jan Halámek, Marcos Pita, Evgeny Katz. “Reversible Gating Controlled by Enzymes at Nanostructured Interface”. Chemical communications, 2010, 46, 2088–2090.

52. Guinevere Strack, Vera Bocharova, Mary A. Arugula, Marcos Pita, Jan Halámek, Evgeny Katz. “Artificial Muscle Reversibly Controlled by Enzyme Reactions”. Journal of physical chemistry letters 2010, 1 (5), 839–843.

51. Tsz Kin Tam, Marcos Pita, Oleksandr Trotsenko, Mikhail Motornov, Ihor Tokarev, Jan Halámek, Sergiy Minko, Evgeny Katz. “Reversible “Closing” of an Electrode Interface Functionalized with a Polymer Brush by an Electrochemical Signal”. Langmuir, 2010, 26 (6), 4506–4513.

50. Tsz Kin Tam, Marcos Pita, Mikhail Motornov, Ihor Tokarev, Sergiy Minko, Evgeny Katz. “Electrochemical Nanotransistor from Mixed Polymer Brush”, Advanced Materials, 2010, 22 (16), 1863-1866.

49. Valeriya Bychkova, Alexey Shvarev, Jian Zhou, Marcos Pita, Evgeny Katz. “Enzyme Logic Gate Associated with a Single Responsive Microparticle: Scaling Biocomputing to Microsize Systems”. Chemical Communications, 2010, 46, 94-96.

48. Tsz Kin Tam, Marcos Pita, Mikhail Motornov, Ihor Tokarev, Sergiy Minko, Evgeny Katz. “Modified Electrodes with Switchable Selectivity for Cationic and Anionic Redox Species”. Electroanalysis, 2010, 22(1), 35-40.

47. Marcos Pita, Guinevere Strack, Kevin MacVittie, Jian Zhou, Evgeny Katz. “Set-Reset Flip-Flop Memory Based on Enzyme Reactions: Towards Memory Systems Controlled by Biochemical Pathways”. Journal of Physical Chemistry B, 2009, 113 (49), 16071–16076.

46. Mary A. Arugula, Jan Halámek, Evgeny Katz, Dmitriy Melnikov, Marcos Pita, Vladimir Privman, Guinevere Strack. “Optimization of Enzymatic Logic Gates and Networks for Noise Reduction and Stability”. IEEE Comp. Soc. Publ. (Los Alamitos, California) 2009, 1-7.
Achieved the “Best Paper Award” by the International Academy, Research, and Industry Association when presented at CENICS 2009 in Sliema, Malta, October 11-16, 2009.

45. M. El-Hilo, J. Al Saie, M.P. Morales, M. Pita. “Simple Technique For Texture Function Analysis in Granular Thin Film Media”. Physica B, 2009, 404, 3991-3994.

44. Jian Zhou, Mary A. Arugula, Jan Halámek, Marcos Pita, Evgeny Katz. “Enzyme-Based NAND and NOR Logic Gates with Modular Design”. Journal of Physical Chemistry B, 2009, 113 (49), 16065–16070.

43. Evgeny Katz, Marcos Pita. “Biofuel Cells Controlled by Logically Processed Biochemical Signals: Towards Physiologically Regulated Bioelectronic Devices”. Concept paper in Chemistry: an European Journal, 2009, 15, 12554-12564.

42. Vladimir Privman, Valber Pedrosa, Dmitriy Melnikov, Marcos Pita, Aleksandr Simonian, Evgeny Katz. “Enzymatic AND-Gate Based on Electrode-Immobilized Glucose-6-Phosphate Dehydrogenase: Towards Digital Biosensors and Biochemical Logic Systems with Low Noise”. Biosensors and Bioelectronics, 2009, 25 (4), 695-701.

41. Cristina Vaz, Susana Campuzano, Víctor M. Fernández, Marcos Pita. “Nanostructured DNA biosensors based on artificial nucleic acid analogues”. Fabrication and Bio-Application of Functionalized Nanomaterials 7, p. 207-228, 2009. X. Wang (ed), Research Signpost, Kerala, India.

40. Mikhail Motornov, Tsz Kin Tam, Marcos Pita, Ihor Tokarev, Evgeny Katz, Sergiy Minko. “Switchable selectivity in gating of ion transport with mixed polyelectrolyte brushes for Smart delivery systems”. Nanotechnology, 2009, 20 (43) 434006-434016.

39. Xuemei Wang, Jian Zhou, Tsz Kin Tam, Evgeny Katz, Marcos Pita. “Switchable Electrode Controlled by Boolean Logic Gates Using Enzymes as Input Signals”. Bioelectrochemistry, 2009, 77(1), 69-73.
38. Tsz Kin Tam, Guinevere Strack, Marcos Pita, Evgeny Katz. “Biofuel Cell Logically Controlled by Antigen-Antibody Recognition: Towards Immune-Regulated Bioelectronic Devices”. Journal of the American Chemical Society, 2009, 131 (33), 11670–11671.

37. Guinevere Strack, Soujanya Chinnapareddy, Dmytro Volkov, Jan Halámek, Marcos Pita, Igor Sokolov, Evgeny Katz. “Logic Networks Based on Immunorecognition Processes”. Journal of Physical Chemistry B, 2009, 113 (35), 12154–12159.

36. Arshak Poghossian, Melina Krämer, Maryam H. Abouzar, Marcos Pita, Evgeny Katz, Michael J. Schöning. “Interfacing of biocomputing systems with silicon chips: Enzyme logic gates based on field-effect devices”. Procedia Chemistry, 2009, 1 (1), 682-685.

35. Dmitriy Melnikov, Guinevere Strack, Marcos Pita, Vladimir Privman, Evgeny Katz. “Analog Noise Reduction in Enzymatic Logic Gates”. Journal of Physical Chemistry B, 2009, 113 (30), pp 10472–10479.

34. Kalayil Manian Manesh, Jan Halámek, Marcos Pita, Jian Zhou, Tsz Kin Tam, Padmanabhan Santhosh, Min-Chieh Chuang, Joshua R. Windmiller, Dewi Abidin, Evgeny Katz and Joseph Wang. “Enzyme Logic Gates for the Digital Analysis of Physiological Level upon Injury”. Biosensors & Bioelectronics, 2009, 24, 3569–3574.

33. Marcos Pita, Tsz Kin Tam, Sergiy Minko and Evgeny Katz. “Dual Magneto-Biochemical Logic Control of Electrochemical Processes Based on Local Interfacial pH Changes” ACS Applied Materials & Interfaces, 2009, 1 (6), pp 1166–1168.

32. Tsz Kin Tam, Marcos Pita, Evgeny Katz. “Enzyme Logic Network Analyzing Combinations of Biochemical Inputs and Producing Fluorescent Output Signals: Towards Multi-Signal Digital Biosensors.” Sensor & Actuators B. Chemical, 2009, 140 (1), 1-4.

31. Marcos Pita, Jian Zhou, Kalayil Manian Manesh, Jan Halámek, Evgeny Katz, Joseph Wang. “Enzyme Logic Gates for Assessing Physiological Conditions During an Injury.” Sensors and Actuators B: Chemical, 2009, 139 (2), 631-636.

30. Ihor Tokarev, Venkateshwarlu Gopishetty, Jian Zhou, Marcos Pita, Mikhail Motornov, Evgeny Katz, Sergiy Minko. “Stimuli-Responsive Hydrogel Membranes Coupled with Biocatalytic Processes”. ACS Applied Materials & Interfaces, 2009, 1 (3), pp 532–536.

29. Jian Zhou, Galina Melman, Marcos Pita, Artem Melman, Evgeny Katz. “Biomolecule Oxidative Damage Activated by Enzyme Logic Systems: Biologically Inspired Approach.” ChemBioChem, 2009, 10 (6), 1084-1090.

28. Tsz Kin Tam, Marcos Pita, Maryna Ornatska, Evgeny Katz. “Biofuel Cell Controlled by Enzyme Logic Network – Approaching Physiologically Regulated Devices”. Bioelectrochemistry, 2009, 76 (1-2), 4-9.

27. Vladimir Privman, Mary A. Arugula, Jan Halámek, Marcos Pita, Evgeny Katz. “Network Analysis of Biochemical Logic for Noise Reduction and Stability: A System of Three Coupled Enzymatic AND Gates”. The Journal of Physical Chemistry B, 2009, 113 (15), 5301–5310.

26. Marina Privman, Tsz Kin Tam, Marcos Pita, Evgeny Katz. “Switchable Electrode Controlled by Biocomputing Network System: Approaching Physiologically Regulated Bioelectronics”. Journal of the American Chemical Society, 2009, 131 (3), 1314–1321. DOI: 10.1021/ja8088108.

25. Liron Amir, Tsz Kin Tam, Marcos Pita, Michael M. Meijler, Lital Alfonta, Evgeny Katz. “Biofuel Cell Controlled by Enzyme Logic Systems”. Journal of the American Chemical Society, 2009, 131 (2), pp 826–832. DOI: 10.1021/ja8076704.

24. Melina Krämer, Marcos Pita, Jian Zhou, Maryna Ornatska, Arshak Poghossian, Michael J. Schöning, Evgeny Katz. “Coupling of Biocomputing Systems with Electronic Chips: Electronic Interface for Transduction of Biochemical Information”. Journal of Physical Chemistry C, 2009, 113 (6), pp 2573–2579.

23. Jian Zhou, Tsz Kin Tam, Marcos Pita, Maryna Ornatska, Sergiy Minko, Evgeny Katz.
“Bioelectrocatylic System Coupled with Enzyme-Based Biocomputing Ensembles Performing Boolean Logic Operations: Approaching “Smart” Physiologically Controlled Bioelectrodes”. ACS Applied Materials & Interfaces, 2009, 1 (1), pp 144–149.

22. Mikhail Motornov, Jian Zhou, Marcos Pita, Ihor Tokarev, Venkateshwarlu Gopishetty, Evgeny Katz, Sergiy Minko. “Integrated Multifunctional Nanosystem from Command Nanoparticles and Enzymes”, Small, 2009, 5 (7), 817-820.

21. Marcos Pita, Evgeny Katz. “Switchable Electrodes: How Can the System Complexity be Scaled Up?” Electroanalysis, 2009, 21(3-5), 252-260.

20. Marcos Pita, Sergiy Minko, Evgeny Katz. “Enzyme-Based Logic Systems and their Applications for Novel Multi-Signal-Responsive Materials”. Journal of Materials Science: Materials in Medicine, 2009, 20 (2), 457-462.

19. Marcos Pita, Melina Krämer, Jian Zhou, Arshak Poghossian, Michael J. Schöning, Víctor M. Fernández, Evgeny Katz. “Optoelectronic Properties of Nanostructured Ensembles Controlled by Biomolecular Logic Systems”. ACS Nano, 2008, 2 (10); 2160-2166.

18. Tam Tsz Kin, Jian Zhou, Maryna Ornatska, Marcos Pita, Sergiy Minko, Evgeny Katz.
“Biochemically Controlled Bioelectrocatalytitic Interface” Journal of the American Chemical Society, 2008, 130 (33); 10888-10889.

17. Mikhail Motornov, Jian Zhou, Marcos Pita,Venkateshwarlu Gopishetty, Ihor Tokarev, Evgeny Katz, Sergiy Minko. ““Chemical Transformers” from Nanoparticle Ensembles Operated with Logic”. Nano Letters, 2008, 8 (9); 2993-2997.
Article highlighted in Nature Nanotechnology Research Highlights:

16. Marcos Pita, Lili Cui, Ravi M. Gaikwad, Evgeny Katz, Igor Sokolov. “High Sensitivity Molecular Detection with Remote ELISA–Type Immunosensing”. Nanotechnology 19 (2008) 375502 (6pp).

15. Vladimir Privman, Guinevere Strack, Dmitry Solenov, Marcos Pita, Evgeny Katz. “Optimization of Enzymatic Biochemical Logic for Noise Reduction and Scalability: How Many Biocomputing Gates Can be Interconnected in a Circuit?”, Journal of Physical Chemistry B, 2008, 112(37); 11777-11784.

14. Cristina Vaz-Dominguez, Susana Campuzano, Olaf Rüdiger, Marcos Pita, Marina Gorbacheva, Sergey Shleev, Víctor M. Fernandez, Antonio L. De Lacey. “Enzymatic electrode for direct electrocatalytic reduction of O2 to H2O with high operational stability and resistance to chloride inhibition”. Biosensors and Bioelectronics, 2008, 24, 531–537.

13. Javier Jiménez, Roman Sheparovych, Marcos Pita, Arántzazu García, Elena Dominguez, Sergiy Minko, Evgeny Katz. “Magneto-induced Self-assembling of Conductive Nanowires for Biosensor Applications”, Journal of Physical Chemistry C, 2008; 112(19); 7337-7344.

12. Tam Tsz Kin, Maryna Ornatska, Marcos Pita, Sergiy Minko, Evgeny Katz. “Polymer Brush-Modified Electrode with Switchable and Tunable Redox Activity for Bioelectronic Applications”, Journal of Physical Chemistry C, 2008; 112(22); 8438-8445.

11. Guinevere Strack, Maryna Ornatska, Marcos Pita, Evgeny Katz. “Biocomputing Security System: Concatenated Enzyme-Based Logic Gates Operating as a Biomolecular Keypad Lock”, Journal of the American Chemical Society, 2008; 130(13); 4234-4235.
Article marked by the editor as top contribution:
Article highlighted in press releases:

10. Guinevere Strack, Marcos Pita, Marina Ornatska, Evgeny Katz. “Boolean Logic Gates Using Enzymes as Input Signals”, Chem. Bio. Chem. 2008, 130 (9), 1260-1266.

9. Marcos Pita, José M. Abad, Cristina Vaz-Dominguez, Carlos Briones, Eva Mateo-Martí, José A. Martín-Gago, María del Puerto Morales, Víctor M Fernández. “Synthesis of Cobalt Ferrite Core / Metallic Shell Magnetic Nanoparticles for the Development of a Specific PNA/DNA Biosensor”, Journal of Colloid Interface Science, 2008, 321, 484-492.

8. Marcos Pita, Evgeny Katz. “Multiple Logic Gates Based on Electrically Wired Surface-Reconstituted Enzymes”. Journal of the American Chemical Society, 2008, 130(1), 36-37.

7. Sergey Vasiylev, Marcos Pita, Evgeny Katz. “Logic Gates Based on Magnetic Nanoparticles Functionalized with a Bioelectrocatalytic System.” Electroanalysis 2008, 1, 22-29.

6. Sergey Shleev, Marcos Pita, Alexander I. Yaropolov, Tautgirdas Ruzgas, Lo Gorton. “Direct heterogeneous electron transfer reactions of Trametes hirsuta laccase at bare and thiol-modified gold electrodes”. Electroanalysis 2006, 18, 1901-1908.

5. Ana Beloqui, Marcos Pita, Julio Polaina, Arturo Martínez-Arias, Olga V. Golyshina, Miren Zumárraga, Michail M. Yakimov, Humberto García-Arellano, Miguel Alcalde, Víctor M. Fernández, Kieran Elborough, José M. Andreu, Antonio Ballesteros, Francisco J. Plou, Kenneth N. Timmis, Manuel Ferrer, and Peter N. Golyshin. “Novel Polyphenol Oxidase Mined From A Metagenome Expresion Library Of Bovine Rumen: Biochemical Properties, Structural Analysis And Phylogenetic Relationships”, Journal of Biological Chemistry 2006, 281, 22933-22942.

4. Marcos Pita, Sergey Shleev, Tautgirdas Ruzgas, Víctor M. Fernández, Alexander I. Yaropolov, Lo Gorton. “Direct heterogeneous electron transfer reactions of fungal laccases at bare and thiolmodified gold electrodes”. Electrochemistry Communications 2006, 8, 747-753.

3. José M. Abad, Marcos Pita, Víctor M. Fernández. “Immobilization of Proteins on Gold Surfaces”. Methods in Biotechnology 22, p. 229-238, 2006. J.M. Guisan (ed), Humana Press Inc., Totowa, NJ.

2. José M. Abad, Stijn F. L. Mertens, Marcos Pita, Víctor M. Fernández, David J. Schiffrin. “Functionalization of Thioctic Acid-Capped Gold Nanoparticles for Specific Immobilization of Histidine-Tagged Proteins”. Journal of the American Chemical Society; 2005, 127(15), 5689-5694.

1. Ana M. Rubio, Marcos Pita, Juan J. Freire. “Cyclization Kinetics of Nondiluted Bond Fluctuation Chains” Macromolecules; 2002, 35, 5681-5687.