Description

BBG liderated by Dr Otero investigates  the application of biocatalysts to develop selective processes of synthesis of chemical products of application in different areas: functional foods, nutraceuticals, sustainable chemistry, biomedicine, phamacy, cosmetics, biodiesel, fine chemicals, etc.

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The research Group of Biocatalysis and Bioenergy (BBG) belongs to the Institute of Catalysis and Petrochemistry, which is part of the Spanish Council for Scientific Research (CSIC), The Institute is one of the research centres included in the field of Chemistry and Chemical Technologies.
The group liderated by Dr Otero investigates the application of biocatalysts to develop selective industrial processes in different areas: functional foods, nutraceuticals, sustainable chemistry, biomedicine, pharmaceutical, cosmetics, biodiesel, fine chemicals, etc.

For the industrial development of new selective biocatalytic processes, BBG investigates immobilized enzymes working in both batch and continuous reactors. Since 1987, the group exploits the  advantages of lipase-catalysed bioprocesses in water and in the presence of organic solvents in the reaction media (selectivity, especificity, etc). BBG investigates the synthesis of compounds used as food additives or emulsifiers: mono-, di- and triacylglycerides, and fatty acid esters of monosaccharides, polyols, amines, alpha-hydroxyacids, biodiesel and phospholipids. The group has wide experience in most of the techniques that are relevant in the field of Biocatalysis: Methodology for the immobilization/stabilization of enzymes, reaction and reactor engineering. The chemical characterization of products and the progress of the enzymatic reactions are followed by TLC, HPLC, gas-liquid
chromatography, NMR and others.

The laboratory of BBG is integrated in the network of laboratories of Madrid+D, running analysis of FAME, glycerides, biodiesel according to directives EN ISO 9001. Analytical methods mostly used in characterization of food-grade fats and oils, and biodiesel are certified by AENOR (European National Agency of Normalization). Determination of FAMEs, oxidative stability, oil composition in terms of mono-, diand triacylglycerol, free fatty acids, water content, density, viscosity, solid fat content, etc are carried out.

BBG group has established fruitful collaborations with the Dept. Chem. Eng. of Wisconsin Univ., the Technological Institute of Veracruz (Mexico) and Korea University, and has participated in different international programs (NATO, EuropeanUnion, Royal Soc. Of UK, Korean KOSEF, Romanian Academy of Sciences, etc). BBG liderates nacional and international scientific consortia for evaluation and demonstrationof functional properties of new oil formulations for patients with obesity, methabolicsyndrome, diabetes, colesterol, hipertension and cancer, and for pregnants, lactants andold citizens.

BBG has developed a process of synthesis of biodiesel from sunflower oil. The processoptimization involves the design of new catalysts more efficient in the alcoholysisreaction, the reactor design and determination of optimal operational conditions. BBGalso organizes monographic courses about synthesis and characterization of biodiesel,methods of characterization of materials, enzymes, biocatalysis, biotransformations and lipids.

Research Lines of BBG:

Enzymatic preparation of fats free of trans fatty acids: margarines and others

Synthesis and characterization of functional lipids and phospholípids, lipids rich or not in  polyunsaturated fatty acids or PUFA (omega-3, omega-6, CLA, etc)

Synthesis and characterization of biodiesel from plant oils, algae, etc

Synthesis and characterization of emulsifiers derived from amines, monosacharides, and sorbitol

Synthesis of a-hydroxyacids, oligosaccharides and others

Identification and extraction of biomass subproducts (antioxidants, vitamins, phospholipids, antimicrobials, glycerides, fatty acids, etc)

Characterization of fats, oils and biodiesel according with European directives:

-Chemical characterization (HPLC-mass, CG-mass, etc for FAME, TAG, DAG, MAG, G, free fatty acids, humidity by Karl Fisher, etc)

-Physical and functional characterization (meeting and crystallization profiles of fats, oxidative stability (Rancimat), viscosity, density, etc

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Facilities & Technologies available in BBG

Extraction & Purification of enzymes

Determination of enzymatic activities

Determination of activities and stabilities of biocatalysts

Improvement of the biocatalyst: Immobilization & modification of enzymes

Analyses of substrates and products according to european directives

Extraction, Separation, & Purification of products mediated or not of a selective biotransformation: products not separable by physical methods such as lipids, phospholipids, antioxidants, etc.

 

Processes catalyzed by enzymes (lipases, proteases, cellulases, phospholipases). Puesta a punto otras por encargo. Ingeniería del medio, de la reacción, del biocatalizador y del reactor para el diseño de un proceso óptimo a medida de cada materia prima (pureza, origen, inhibidores, etc).

Optimización y Desarrollo de procesos (incluye escalado y diseños a escala piloto) de obtención de compuestos para química fina, aditivos alimentarios, emulgentes, antioxidantes, cosméticos, fármacos quirales, etc.

Procesos desarrollados/patentados por el BBG

Síntesis de selectiva de emulgentes de grado alimentario (derivados de monosacáridos y polioles)

Síntesis de grasas semisólidas libres de ácidos trans

Síntesis selectiva de lípidos estructurados (Light, ricos en PUFA tipo omega-3..)

Síntesis selectiva de fosfolípidos estructurados (Light, ricos en PUFA tipo omega-3..)

Producción de biodiesel a partir de aceites de 1ª y última generación. Dos procesos: en tanque agitado y sistema en continuo.

Caracterización química, física y funcional de todas las materias primas y todos los productos

Cromatografía de gases-masas, de líquidos-masas, RMN, IR, estabilidad oxidativa, perfiles de fusión y cristalización, plasticidad de grasas, viscosidad, contenido en agua por Karl Fisher, densidad, etc.

Caracterización de lípidos y biodiesel conforme a normativa europea (composición en FA, glicéridos, FAMEs y FAEEs, estabilidad oxidativa y acidez libre).

Con grupos colaboradores: Pruebas funcionales en animales y humanos (colesterol, diabetes, hipertensión, síndrome metabólico, cáncer, etc).

People

Otero Hernández, Cristina – Group leader

Publications

PROLONGATION OF SECONDARY DRYING STEP OF PHOSPHOLIPID LYOPHILIZATION GREATLY IMPROVES ACIDOLYSIS REACTIONS CATALYZED BY IMMOBILIZED LECITASE ULTRA

Year: 2020
Authors: Carlos Manuel Verdasco Martín, Roberto Fernandez-Lafuente, Cristina Otero Hernández
 Enzyme and Microbial Technology

Rapid and high yield production of phospholipids enriched in CLA via acidolysis: the critical role of the enzyme immobilization protocol

Year: 2019
Authors: Carlos Manuel Verdasco Martín, Roberto Fernandez-Lafuente, Cristina Otero Hernández
Food Chemistry

Selective synthesis of partial glycerides of conjugated linoleic acids via modulation of the catalytic properties of lipases by immobilization on different supports

Year: 2018
Authors: Carlos Manuel Verdasco Martín, Roberto Fernandez-Lafuente, Cristina Otero Hernández
FOOD CHEMISTRY

Optimization of the coating of octyl-CALB with ionic polymers to improve stability and decrease enzyme leakage

Year: 2018
Authors: Cristina Otero Hernández, Roberto Fernandez-Lafuente
Biocatalysis and Biotransformations

Patentes

Extracto acuoso con propiedades anti-hipertensivas, anti-hiperlipidémicas, anti-hipercolesterolémicas y antioxidantes.

2018

El CSIC ha desarrollado un método de extracción de biocomponentes de espirulina que poseen gran actividad anti-hipertensiva, anti-hiperlipidémica y antioxidante para su uso en la industria alimentaria, cosmética o farmacéutica.
Se buscan empresas interesadas en la licencia de la patente para el desarrollo de estos biocomponentes como productos comercializables.