Articles

151. Palomo,* J.M., E.P. Urriolabeitia. Protein-Pd Complexes Conjugates as Artificial Enzymes.
‎Gen. Chem. 2020, 6, 190030.

152. Losada-Garcia, N., Rodriguez-Otero, A., Palomo,* J.M. Tailorable synthesis of heterogeneous enzyme–copper nanobiohybrids and their application in the selective oxidation of benzene to phenol.
Catal.Sci.Technol., 2020, 10, 196-206.

Graphical abstract: Tailorable synthesis of heterogeneous enzyme–copper nanobiohybrids and their application in the selective oxidation of benzene to phenol

152. Losada-Garcia, N., Rodriguez-Oliva, I., Simovic, M., Bezbradica, D.I.,  Palomo,* J.M. New Advances in Fabrication of Graphene Glyconanomaterials for Application in Therapy and Diagnosis.
ACS Omega, 2020, 5,4362-4369.

153.  Palomo,* J.M.  Synthetic complexity created by lipases.
Nature Catal., 2020, 3, 335-336.

 

154. Losada-García, N., Rodriguez-Otero, A., Palomo , J.M., Fast Degradation of Bisphenol A in water by nanostructured CuNPs@CAL-B biohybrid catalyst. Nanomaterials. 2020, 10, 7.

155. Benavente,R., Lopez-Tejedor,D., Morales, M.P,   Perez-Rizquez, C.,  Palomo J.M. The enzyme-induced formation of iron hybrid nanostructures with different morphologies.
Nanoscale, 2020,12, 12917-12927

156. McCarthy, C., Losada-García, N., Palomo, J.M., Direct synthesis of phenols from phenylboronic acids in aqueous media catalyzed by a Cu(0)-nanoparticles biohybrid.
Chemistry select 2020, 5, 7492-7496.

157. Losada-García, N., Rodriguez-Otero, A., Palomo, J.M., High degradation of trichloroethylene in water by nanostructured MeNPs@CALB biohaybrid catalysts.
Catalyst. 2020. 2020, 10(7), 753

158. Iron Catalysis Design and Applications. Edited By: Jose M Palomo.
Catalytic Science Series: Volume 19. Pages: 320 https://doi.org/10.1142/q0283 | March 2021. 

159. Johana Jaramillo, Ivan Rodriguez-Oliva, Olga Abian and Jose M. Palomo. Specific chemical incorporation of L-DOPA and functionalized
L-DOPA-hyaluronic acid in Candida antarctica lipase: creating potential mussel-inspired.
SN Applied Sciences, 2020.DOI: https://doi.org/10.1007/s42452-020-03545-w

145. Fernández-Lodeiro, A.; Djafari, J.; Lopez-Tejedor, D.; Perez-Rizquez, C.; Rodríguez-González, B.; Capelo, J.L.; Palomo*, J.M.; Lodeiro, C., Fernández-Lodeiro, J. Highly accessible aqueous synthesis of well-dispersed dendrimer type platinum nanoparticles and their catalytic applications.
‎Nano Res. 2019, 12, 1083

146.Perez-Rizquez, C.; Lopez-Tejedor, D.; Plaza-Vinuesa, L.; de las Rivas, B.; Muñoz, R.; Cumella, J.; Palomo*, J.M. Chemical Modification of Novel Glycosidases from Lactobacillus plantarum Using Hyaluronic Acid: Effects on High Specificity against 6-Phosphate Glucopyranoside.                                                                                                                  Coatings 2019, 9, 311

147. Perez-Rizquez, C.;Rodriguez-Otero, A.;Palomo,* J.M., Combining enzymes and organometallic complexes: novel artificial metalloenzymes and hybrid systems for C-H activation chemistry. 
‎Org.Biomol.Chem. 2019,17, 7114-7123

Graphical abstract: Combining enzymes and organometallic complexes: novel artificial metalloenzymes and hybrid systems for C–H activation chemistry

148. Palomo,* J.M., Nanobiohybrids: a new concept for metal nanoparticles synthesis.
‎Chem. Commun. 2019. 55, 9583-9589.

Graphical abstract: Nanobiohybrids: a new concept for metal nanoparticles synthesis

149 Losada-Garcia, Noelia; Berenguer-Murcia, Angel; Cazorla-Amorós, Diego; Palomo,* J.M., Efficient production of multi-layer graphene from graphite flakes in water by lipase-graphene sheets conjugation.
‎Nanomaterials. 2019.9(9), 1344

First in ChemRvix: Losada-Garcia, Noelia; Berenguer-Murcia, Angel; Cazorla-Amorós, Diego; Palomo,* J.M.,
Green Exfoliation of Graphite Flakes to Efficiently Synthesize Biographene.

150. Perez-Rizquez, C., Abian, O., Palomo,* J.M., Site-Selective modification of Tryptophan and Protein Tryptophan residues through PdNPs bionanohybrid-catalysed C-H Activation in Aqueous media.
‎Chem. Commun. 2019, 55, 12928-12931.

Graphical abstract: Site-selective modification of tryptophan and protein tryptophan residues through PdNP bionanohybrid-catalysed C–H activation in aqueous media

137. Lopez-Tejedor, D., Palomo,* J.M., Efficient purification of a highly active H-subunit of tyrosinase from Agaricus bisporus.
‎Protein Expr. Purif . 2018, 145, 64-70.

Fig. 11. SDS-PAGE analysis of the two-step chromatography purification cascade

138. Romero, O, de las Rivas, B., Lopez-Tejedor,D.,Palomo,* J.M., Effect of Site-Specific Peptide-Tag Labeling on the Biocatalytic Properties of Thermoalkalophilic Lipase from Geobacillus thermocatenulatus.
ChemBioChem. 2018. 19, 369-378.

image

139. Lopez-Tejedor, D., Benavente, R., Palomo,* J.M., Iron Nanostructured catalysts: Design and applications.
‎Catal Scien.Technol 2018, 8, 1754-1776.

Graphical abstract: Iron nanostructured catalysts: design and applications

140. Benavente, R., Lopez-Tejedor, D., Palomo,* J.M.,
Synthesis of a superparamagnetic ultrathin FeCO3 nanorods–enzyme bionanohybrid as a novel heterogeneous catalyst
‎ChemCommun 2018, 54, 6256-6259.

Graphical abstract: Synthesis of a superparamagnetic ultrathin FeCO3 nanorods–enzyme bionanohybrid as a novel heterogeneous catalyst

Inside Cover ChemComm Issue

141. Benavente, R., Lopez-Tejedor, D., Perez-Rizquez, C., Palomo,* J.M.,
Ultra-Fast Degradation of p-Aminophenol by a Nanostructured Iron Catalyst
‎Molecules 2018, 23, 2166.

142. O´Brien, P., Lopez-Tejedor, D., Benavente, R., Palomo,* J.M.,
Pd nanoparticles-Polyethylenemine-Lipase Bionanohybrids as heterogeneous catalysts for selective oxidation of aromatic alcohols
‎ChemCatChem 2018, 10, 5006–5013.

143. Lopez-Tejedor, D., de las Rivas, B., Palomo,* J.M.,
Ultra-small Pd(0) nanoparticles into a designed semisynthetic lipase: an efficient and recyclable heterogeneous biohybrid catalyst for the Heck
reaction under mild conditions
‎Molecules 2018, 23(9), 2358.

144. Mateo, C., Palomo,* J.M.,
Asymmetric and Selective Biocatalysis

‎Catalysts 2018,8 (12), 588.

133. Filice, M., Aragon C., Mateo C., Palomo,* J.M.,
Enzymatic transformations in food chemistry
Curr. Org. Chem. 2017, 21 (2), 139-148

134. Callaghan, C., Redmond, M., Alnoch, R.C., Mateo, C., Filice, M.,Palomo,* J.M.,
Biocatalytic process optimization for production of high-added value 6-O-hydroxy and 3-O-hydroxy glycosyl building blocks
ChemCatChem. 2017.9, 2536-2543.

135.Palomo,* J.M.,
Semisynthetic Enzymes by Protein–Peptide Site-Directed Covalent Conjugation: Methods and Applications
Methods in Enzymology. 2017. 590, 305-316.

136. Filice, M., Molina M., Benaiges M.D., Abian, O., Valero F, Palomo,* J.M.
Solid-surface activated recombinant Rhizopous oryzae lipase expressed in Pichia Pastoris and chemically modified variants as efficient catalysts in the synthesis of hydroxy monodeprotected glycals.
Catal. Sci. Technol. 2017, 7, 1766-1775.

Graphical abstract: Solid-surface activated recombinant Rhizopous oryzae lipase expressed in Pichia pastoris and chemically modified variants as efficient catalysts in the synthesis of hydroxy monodeprotected glycals

125. Aragon C., Palomo, J.M., Filice, M.,Mateo C. Immobilization of Aldolase for C-C Bond Formation
Curr. Org. Chem. 2016, 20,1243-1251.

126. Cuenca, T., Filice, M., Palomo*, J.M.Palladium nanoparticles enzyme aggregate (PANEA) as efficient catalyst for Suzuki-Miyaura reaction in aqueous media
Enzyme Microb. Technol. 2016, 95,242-247

Image for unlabelled figure

127. Palomo*, J.M., Filice, M.Biosynthesis of Metal Nanoparticles: Novel Efficient Heterogeneous Nanocatalysts
Nanomaterials 2016, 6(5), 84,

128.Brabcova, J., Blazek, J. Krecmerova, M., Vondrášek J, Palomo*, J. M., Zarevucka, M.Regioselective palmitoylation of 9-(2,3-dihydroxypropyl)adenine catalysed by a glycopolymer-enzyme conjugate.                                                                                                    Molecules.2016,21(5), 648.

129. Rivero C.W.,Palomo,* J.M., Covalent immobilization of Candida rugosa lipase at alkaline pH and their application in the regioselective deprotection of per-O-acetylated thymidine transformations
Catalysts. 2016,6(8), 115

130. Dunne, A.,Palomo,* J.M., Efficient and green approach for the complete deprotection of O-acetylated biomolecules.
RSC advances. 2016,6, 88974-88978.

Graphical abstract: Efficient and green approach for the complete deprotection of O-acetylated biomolecules

131. Alnoch, R., Rodrigues de Melo, R., Palomo, J.M.,Maltempi de Souza, E., Krieger,N., Mateo C.New Tailor-Made Alkyl-Aldehyde Bifunctional Supports for Lipase Immobilization.
Catalysts. 2016,6, 191.

132. Neville, A., Iniesta J., Palomo,* J.M., Design of heterogeneous Hoveyda-Grubbs Second-generation catalyst-lipase conjugates.
Molecules.2016, 21, 1680; doi:10.3390/molecules21121680

120. Palomo*, J.M, .Filice, M.
New emerging bio-catalysts design in biotransformations.
Biotechnol Adv.2015,33, 605-613.

121. Branco, R. V., Gutarra, M. L. E., Guisan, J. M., Freire, D. M. G., Almeida R. V., Palomo, J.M,.
Improving the Thermostability and Optimal Temperature of a Lipase from the Hyperthermophilic Archaeon Pyrococcus furiosus by Covalent Immobilization.
BioMed Research International, vol. 2015, Article ID 250532, 8 pages, 2015.

122. Filice, M.,Romero O, Gutierrez-Fernandez, J., de las Rivas B., Hermoso J.A.,Palomo, J.M.
Synthesis of a heterogeneous artificial metallolipase with chimeric catalytic activity
Chem. Commun .2015, 51, 9324-9327.

Graphical abstract: Synthesis of a heterogeneous artificial metallolipase with chimeric catalytic activity

123. Quintana, P.G., Guillen, M., Marciello, M., Valero, F; Palomo, J.M., Baldessari, A.
Enzyme-catalyzed preparation of chenodeoxycholic esters by an immobilized heterologous Rhizopus oryzae lipase
J. Mol. Catal B: Enzym.2015,118,36-42

image

124. Filice, M.,Romero O, Aires, A., Guisan, J.M., Rumbero, A., Palomo*, J.M.
Preparation of an immobilized lipase-palladium artificial metalloenzyme as catalyst in Heck reaction: role of the solid-phase
Adv. Synth. Catal. 2015,357, 2687-2696.

116. Filice, M, Palomo*, J. M.

Cascade reactions catalyzed by bionanostructures.

ACS Catalysis. 2014, 4, 1588-1598.

Abstract Image

117.Brabcova, J.,Carrasco-Lopez, C., Bavaro T., Hermoso, J.A., Palomo*, J. M.
Escherichia coli LacZ β-galactosidase inhibition by monohydroxy acetylated glycopyranosides: Role of the acetyl groups
J Mol Catal B: Enzym. 2014, 107, 31-38.

Full-size image (24 K)

118. Palomo*, J. M.
Solid-phase peptide synthesis: an overview focused on the preparation of biologically relevant peptides.
RSC Advances. 2014, 4, 32658-32672.

Graphical abstract: Solid-phase peptide synthesis: an overview focused on the preparation of biologically relevant peptides

119. Filice, M., Romero O, Abian, O., de las Rivas B., Palomo*, J.M.
Low ionic liquids concentration in water: a green and simple approach to improve activity and selectivity of lipases.
RSC Advances.2014, 4, 49115-49122.

Graphical abstract: Low ionic liquid concentration in water: a green and simple approach to improve activity and selectivity of lipases

107.Godoy, C., de las Rivas, B., Mateo, C., Fernandez-Lorente, G; Guisan, J. M., Palomo*, J.M.
Changes on enantioselectivity of a genetically modified thermophilic lipase by site-directed oriented immobilization
J Mol Cata B: Enzym. 2013, 87,121-127.

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108.de Lima, L.N., Aragon, C.C., Mateo, C., Palomo, J.M, Giordano, R.L.C., Tardioli, P.W., Guisan, J.M., Fernandez-Lorente, G.
Immobilization and stabilization of a bimolecular aggregate of the lipase from Pseudomonas fluorescens by multipoint covalent attachment
Process Biochem. 2013, 48, 118-123.

109. Romero O, Rivero C.W., Guisan, J.M., Palomo*, J. M.
Novel enzyme-polymer conjugates for biotechnological applications
PeerJ :e27 http://dx.doi.org/10.7717/peerj.27. 2013

110. Palomo*, J. M.
Editorial. Hot Topic: Biocatalysis for Organic Synthesis
Curr.Org Chem. 2013,17, 671.

111. Palomo*, J. M.
Click-chemistry in Biocatalysis
Curr.Org Chem. 2013, 17, 691-700.

112 .Brabcova, J., Filice, M. , Gutarra, M., Palomo*, J. M.
Synthesis of enantiopure drugs and drug intermediates by immobilized lipase-catalysis
Curr. Bio.Compd. 2013, 9, 113-136.

113 .Filice, M., Marciello ,M., Morales, M.P., Palomo*, J. M.
Synthesis of heterogeneous enzyme-metal nanoparticle biohybrids in aqueous media and their applications in
C-C bond formation and tandem catalysis
Chem. Commun. 2013, 49 (61), 6876-6878.

Journal cover: Chemical Communications Graphical abstract: Synthesis of heterogeneous enzyme–metal nanoparticle biohybrids in aqueous media and their applications in C–C bond formation and tandem catalysis

114.Palomo*, J. M., Filice, M. ,Romero O., Guisan J.M.
Improving lipase activity by immobilization and post-immobilization strategies
Methods in Molecular Biology 2013, 1051, 255-273.

115.Brabcova, J.,Demianova, Z., Vondrasek, J. Jagr, M., Zarevucka, M., Palomo*, J. M.
Highly selective purification of three lipases from Geotrichum candidum 4013 and their characterization and biotechnological applications
J Mol Cata B: Enzym.2013, 98, 62-72.

98. Fernandez-Lorente, G; Betancor, L; Carrascosa, A.V.; Palomo*, J. M., Guisan, J. M.
Modulation of the selectivity of immobilized lipases by chemical and physical modifications: release of omega-3 fatty acids from fish oil.
J. Am oil Food Chem. 2012, 89, 97-102

99. Filice M, Palomo*, J.M.
Monosaccharide derivatives as central scaffolds in the synthesis of glycosylated drugs
RSC Advances. 2012, 2, 1729-1742
. Highlight in ChemInform 2012 43 (1)

Journal Cover:RSC Adv., 2012, 2, 1729-1742 Graphical abstract: Monosaccharide derivatives as central scaffolds in the synthesis of glycosylated drugs

100. Palomo*, J. M., Guisan, J.M.
Different strategies for hyperactivation of lipase biocatalysts
Methods in Molecular Biology2012, 861, 329-341.

101. Marciello, M., Filice M, Palomo*, J. M.
Different strategies to enhance activities of lipase catalysts
Catal Sci.Technol. 2012, 2, 1531-1543. Highlight in ChemInform 2012 43 (42)

Journal Cover:Catal. Sci. Technol., 2012, 2, 1531-1543 Graphical abstract: Different strategies to enhance the activity of lipase catalysts

102. Quintana, P.G., Guillen, M., Marciello, M., Valero, F, Palomo, J. M., Baldessari, A.
Immobilized heterologous Rhizopus oryzae lipase as an efficient catalyst in the acetylation of cortexolone
Eur.J.Org.Chem., 2012, 23, 4306-4312

103.Romero O, Filice, M., de las Rivas B., Carrasco-Lopez, C., Klett, J., Morreale, A., Hermoso, J.A., Guisan, J.M., Abian, O., Palomo*, J.M.
Semisynthetic Peptide-Lipase Conjugates for Improved Biotransformations
Chem.Commun.2012, 72, 9053-9055
. Highlight inChemInform2012 43 (50)

Journal Cover:Chem. Commun., 2012, 48, 9053-9055 Graphical abstract: Semisynthetic peptide–lipase conjugates for improved biotransformations

104.Filice M,Guisan, Jose M., Terreni, M., Palomo*, J. M.
Regioselective monodeprotection of peracetylated carbohydrates
Nature Protoc.2012, 7, 1783-1796

105. Palomo*, J. M.
Click reactions in protein chemistry: From the preparation of semisynthetic enzymes to new click enzymes
Org Biomol.Chem. 2012, 10 , 9309-9318
(Inside front cover)

Graphical abstract: Inside front cover Graphical abstract: Click reactions in protein chemistry: from the preparation of semisynthetic enzymes to new click enzymes

106. Cabrera Z, Palomo*, J. M.
Enzymatic Desymmetrization of Prochiral Molecules
Curr. Org. Synth., 2012, 9, 791-805

92. Acosta, A.; Filice, M.; Fernandez-Lorente,G., Palomo*, J. M, Guisan, J. M.
Kinetically controlled synthesis of monoglyceryl esters from chiral and prochiral acids methyl esters catalyzed by immobilized Rhizomucor miehei lipase.
Bioresource. Technol. 2011, 102, 507-512.

93.Godoy, C.; Fernandez-Lorente, G; de las Rivas, B.; Filice, M.,Guisan, J. M., Palomo*, J.M.
Medium engineering on modified Geobacillus thermocatenulatus lipase to prepare highly active catalysts.
J. Mol. Cat B: Enzym. 2011, 70,144-148.

94. Gutarra M.L, Mateo, C., Freire, D.M.G., Torres, F.A.G., Castro, A.M., Guisan, J.M.,Palomo*, J. M.
Oriented Irreversible Immobilization of a glycosylated Candida antarctica B lipase on heterofunctional organoborane-aldehyde support.
Catal Sci.Technol. 2011,1, 260-266.

Journal Cover:Catal. Sci. Technol., 2011, 1, 260-266 Graphical abstract: Oriented irreversible immobilization of a glycosylated Candida antarctica B lipase on heterofunctional organoborane-aldehyde support

95. Filice, M.,Romero, O, Guisan, J.M., Palomo*, J. M.
Trans,trans-2,4-hexadiene incorporation on enzymes for site-specific immobilization and fluorescent labeling.
Org Biomol.Chem. 2011,9, 5535-5540.

Journal Cover:Org. Biomol. Chem., 2011, 9, 5535-5540 Graphical abstract: trans,trans-2,4-Hexadiene incorporation on enzymes for site-specific immobilization and fluorescent labeling

96. Gutarra M.L, Romero O., Abian, O., Torres, F.A.G., Freire, D.M.G., Castro, A.M., Guisan, J. M.,Palomo*, J.M.
Enzyme surface glycosylation on the solid phase: improve activity and selectivity of Candida antarctica lipase B.
ChemCatChem. 2011, 3, 1902-1910.

Cover image for Vol. 3 Issue 12 Link to full-size graphical abstract

97. Cabrera Z, Palomo*, J.M.
Enantioselective Desymmetrization of prochiral diesters catalyzed by immobilized Rhizopus oryzae lipase.
Tetrahedron: Asymmetry. 2011, 22, 2080-2084.

Cover image image

85. Volpato, G., Filice, M., Ayub, M.A.Z., Guisan, J. M., Palomo*, J.M.
Single-step Purification of different Lipases from Staphylococcus warneri
J. Cromatogr. A. 2010, 1217, 473-478.

Cover image

86.Godoy, C.; de las Rivas, B.; Filice, M., Fernández-Lorente,G.,Guisan, J. M., Palomo*, J.M.
Enhanced activity of an immobilized lipase promoted by site-directed chemical modification with polymers
Process Biochem. 2010,45, 534-541.

Cover image

87. Filice, M., Guisan, J. M., Palomo*, J.M.
Recent Trends in regioselective Protection and deprotection of monosaccharides
Curr. Org Chem. 2010,14, 516-532. Highlights in ChemInform 2010 41 (39)

88.Cabrera,Z.; Gutarra M.L, Guisan, J.M.,Palomo*, J.M.
Highly enantioselective biocatalysts by coating immobilized lipases with polyethyleneimine
Catal Commun. 2010,11,964-967.

Cover image

89. Filice, M.,Guisan, Jose M., Palomo*, J.M.
Effect of Ionic Liquids as additives in the catalytic properties of different immobilized preparations of Rhizomucor miehei Lipase in the Hydrolysis of Peracetylated Lactal.
Green Chem. 2010,12, 1365-1369.

90. Palomo*, J.M.
Diels-Alder cycloaddition in protein chemistry.
Eur. J. Org Chem.2010,33,6303-6314.
Highlights in ChemInform 2011 42 (11)

European Journal of Organic Chemistry

78. Filice, M; Bavaro T.; Fernandez-Lafuente,R.; Pregnolato M.; Guisan, J. M; *Palomo, J.M, Terreni,  M.
Chemo-Biocatalytic Regioselective Synthesis of Different Deprotected Monosaccharides.
Catal. Today . 2009, 140,11-18.

79. Cabrera,Z.; Fernández-Lorente,G., Fernandez-Lafuente,R.; Guisan, J. M.;Palomo*, J.M.
Enhancement of Novozym-435 catalytic properties by physical or chemical modification.
Process Biochem. 2009,44,226-231.

80. Palomo*, J.M.
Modulation of enzymes selectivity via immobilization.
Curr. Org. Synth. 2009, 6,1-14

81. Carrasco-López, C.; Godoy, C.; de las Rivas, B.; Fernandez-Lorente, G.; Palomo, J.M., Guisán, J. M., Fernández-Lafuente, R.; Martínez-Ripoll M., Hermoso, J.A.
Activation of bacterial thermoalkalophilic lipases is spurred by dramatic structural rearrangements.
J. Biol.Chem.2009, 284,4365-4372.

82. Cabrera,Z.; Fernández-Lorente,G., Fernandez-Lafuente,R.; Palomo*, J.M; Guisan, J.M.
Novozym 435 displays very different selectivity compared to Candida antarctica B lipase adsorbed on standard hydrophobic supports.
J.Mol Cat B: Enzym 2009,57,171-176.

83.Rodrigues, D.S., Mendes, A.A., Filice, M., Guisan, J. M., Fernandez-Lafuente, R., Palomo*, J.M.
Different derivatives of a lipase display different regioselectivity in the monohydrolysis of per-O-acetylated 1-O-substituted-β-galactopyranosides
J.Mol Cat B: Enzym.2009,58,36-40.

84. Filice, M., Vanna, R., Terreni, M., Guisan, J. M., Palomo*, J.M.
Lipase-Catalyzed Regioselective One-step Synthesis of Penta-O-acetyl-3-hydroxy-Lactal.
Eur. J. Org Chem.2009,20,3327-3329.

European Journal of Organic Chemistry

69.Fernandez-Lorentes, G.; Filice, M; Terreni,  M.; Guisan, J. M; Fernandez-Lafuente,R., *Palomo, J. M.
Lecitase® ultra as regioselective biocatalyst in the hydrolysis of fully protected carbohydrates.Strong modulation by using different immobilization protocols.
J. Mol.Cat B: Enzym. 2008, 51,110-117

70. Filice, M, Ubiali, D., Fernandez-Lafuente,R., Fernandez-Lorente, G., Guisan J. M, *Palomo, J. M, Terreni,  M.
A Chemo-Biocatalytic approach in the synthesis of β-O-Naphtylmethyl-N-Peracetylated Lactosamine.
J. Mol.Cat B: Enzym
. 2008, 52-53, 106-112.

71. Fernández-Lorente,G., Cabrera,Z.; Godoy, C.; Fernandez-Lafuente,R.; *Palomo, J. M, Guisan, J.M.
Interfacially activated Lipases against hydrophobic supports: effect of the support nature on the biocatalytic properties
Process Biochem. 2008, 43, 1061–1067.

72. Cabrera,Z.; Fernández-Lorente,G., Palomo, J. M, Fernandez-Lafuente,R.; Guisan, J.M.
Asymmetric hydrolysis of di-methyl ester of phenylglutaric acid catalyzed by Lecitase Ultra®. Effect of the immobilization protocol on its catalytic properties.
Enzyme Microb Technol. 2008, 43, 531–536.

73. Filice, M; Palomo, J. M, Bonomi, P.; Bavaro T.; Fernandez-Lafuente,R.; Guisan, J.M; Terreni,  M.
Preparation of linear oligosaccharides by a simple monoprotective chemoenzymatic approach
Tetrahedron. 2008,64, 9286-9292.

Cover image Full-size image (15 K)

74.Fernandez-Lorente, G., Godoy, C., Mendes, A. A., Lopez-Gallego,F, Grazu V,  de las  Rivas  B., Palomo J.M., Hermoso, J., Fernandez-Lafuente , R, Guisan J.M.
Solid-Phase Chemical Amination of Bacillus thermocatenulatus lipase to improve its stabilization via covalent immobilization on highly activated glyoxyl-agarose
Biomacromolecules. 2008,9, 2553–2561.

75. Palomo, J. M.
Lipases Enantioselectivity Alteration by Immobilization Techniques
Curr. Bio.Comp..
2008,4, 126-138.

76. Mendes, A.A., Rodrigues, D. S., Filice, M., Fernandez-Lafuente, R., Guisan, J. M., *Palomo,J.M.
Regioselective monohydrolysis of per-O-acetylated 1-O-substituted-β-glucopyranosides catalyzed by immobilized lipases.
Tetrahedron. 2008,64,10721-10727.

Cover image Full-size image (19 K)

77.Carrasco-López, C.; Godoy, C.; de las Rivas, B.; Fernandez-Lorente, G.; Palomo, J. M., Guisán, J. M., Fernández-Lafuente, R.; Martínez-Ripoll M., Hermoso, J.A.
Crystallization and preliminary X-ray diffraction studies of the BTL2 lipase from extremophilic microorganism Bacillus thermocatenulatus.
Acta Cryst. Sect F Struct Biol Cryst Commun.2008, 64, 1043–1045.

57. Palomo, J.M.; Segura, Rosa L.; Fernández-Lorente, G.; Guisán, J.M.; Fernández-Lafuente,R.
Glutaraldehyde modification of lipases adsorbed on aminated supports: A simple way to improve their behaviour as enantioselective biocatalyst.
Enzyme Microb Technol. 2007, 40,704-707.

58. Cabrera, Z.; Palomo, J. M.; Fernández-Lorente, G.; Guisán, J.M.; Fernández-Lafuente,R.
Partial and Enantioselective Asymmetric Hydrolysis of diethyl phenylmalonate by immobilized preparations of lipase from Thermomyces lanuginosa.
Enzyme Microb technol.
2007,40,1280-1285.

59. Cabrera, Z.; Lopez-Gallego, F.; Fernández-Lorente, G.; Palomo, J. M.; Montes, T.; Grazu, V.; Guisán, J.M.; Fernández-Lafuente,R.
Asymmetric hydrolysis of dimethyl  phenylmalonate by immobilized preparations of Penicillin G. acylase from e. coli.
Enzyme Microb Technol , 2007, 40,997-1000.

60.Fernández-Lorente, G.;Palomo, J. M.; Cabrera, Z.; Fernández-Lafuente,R.; Guisán, J.M.
Improved catalytic properties of  immobilized lipases by the presence of very low concentrations  of detergents in the reaction medium.
Biotechnol. Bioeng
. 2007,  97,242-250.

61. Mateo C.; Grazu, V.; Palomo J. M.;Lopez-Gallego, F.; Fernandez-Lafuente, R.; Guisán, J.M.
Immobilization of Enzymes on Heterofunctional Epoxy Supports.
Nature protocols. 2007, 2,1022-1033.

62. Palomo, J. M.; Fernández-Lorente, G.; Fernández-Lafuente, R.;Guisán, J.M.
Modulation of the immobilized lipase enantioselectivity via chemical amination.
Adv. Synth Catal.2007, 349,1119-1127.

Advanced Synthesis & Catalysis

63.Fernández-Lorente, G.; Palomo, J. M.; Guisán, J.M. ; Fernández-Lafuente, R.
Effect of the immobilization protocol in the activity, stability and enantioslectivity of LECITASE® ULTRA.
J. Mol.Cat B: Enzym. 2007, 47,99-104.

64.Mateo C.; Palomo J. M.; Fernández-Lorente, G.; Fernandez-Lafuente, R.; Guisán, J.M.
Improvement of enzyme activity, stability and selectivity via immobilization techniques.
Enzyme Microb Technol.
2007,40 1451-1463.

65. Palomo, J. M*; Filice, M; Fernandez-Lafuente,R.; Terreni,  M.; Guisan, Jose M.
Regioselective hydrolysis of peracetylated β-monosaccharides by immobilized lipases. Key Role of the immobilization protocol.
Adv. Synth Cat
. 2007,349,1969-1976

Advanced Synthesis & Catalysis

66.Fernández-Lorente,G., Palomo, J. M, Cabrera,Z.; Guisan, Jose M Fernandez-Lafuente,R.
Specificity enhancement towards hydrophobic substrates by immobilization of lipases by interfacial activation on hydrophobic supports.
Enzyme  Microb. Technol, 2007,41,565-569.

67.Filice, M; Fernandez-Lafuente,R.; Terreni,  M.; Guisan, Jose M, Palomo, J. M*.
Screening of Lipases for Regioselective Hydrolysis of peracetylated β-Monosaccharides.
J. Mol.Cat B: Enzym
. 2007,49,12-17.

68.Mateo C.; Grazu, V.; Pessela B.C.C., Montes, T., Palomo, J.M., Torres, R., Lopez-Gallego, F.; Fernandez-Lafuente, R.; Guisán, J.M.
Advances in the design of new epoxy supports for enzyme immobilization-stabilization.
Biochem.  Soc. Trans.2007,35, 1593–1601.

41.de Araújo A.D.; Palomo, J.M.; Cramer J.; Köhn,M.; Schröder,H , Wacker,R.; Niemeyer , C.; Alexandrov,K.; Waldmann H.
Diels-Alder Ligation and Immobilization of Peptides and Proteins.
Angew. Chem. 2006, 118,302-307.

42.de Araújo A.D.; Palomo, J. M.; Cramer J.; Köhn,M.; Schröder,H , Wacker,R.; Niemeyer , C.; Alexandrov,K.; Waldmann H.
Diels-Alder Ligation and Immobilization of Peptides and Proteins.
Angew. Chem. Int. Ed. 2006,45,296-301. Highlights in Nature Methods

Angewandte Chemie International Edition

43.Palomo, J. M.; Lumbierres, M.; Waldmann ,H.
Efficient Solid-phase synthesis of lipopeptides employing the Ellman sulfonamide linker.
Angew. Chem. 2006, 118, 491-495.

44.Palomo, J. M.; Lumbierres, M.; Waldmann ,H.
Efficient Solid-phase synthesis of lipopeptides employing the Ellman sulfonamide linker.
Angew. Chem. Int. Ed. 2006, 45, 477-481.

Angewandte Chemie International Edition

45.Nicolini, C.; Baranski, J.; Schlummer, S.; Palomo, J.; Lumbierres-Burgues, M.; Kahms, M.; Kuhlmann, J.; Sanchez,J.; Gratton, E.; Waldmann, H.; Winter, R.
Visualizing association of N-Ras in lipid microdomains with two-photon fluorescence excitation and atomic force microscopy the influence of domain structure and interfacial adsorption .
J.Am.Chem.Soc.2006, 128, 192-201.

46.Palomo, J.M.; Segura, R.L.; Fuentes, M.; Ortiz, C.; Guisán, J.M.; Fernández-Lafuente,R
Unusual enzymatic resolution of racemic glycidyl-butyrate for the production of S-glycidyl derivatives.
Enzyme Microb Technol. 2006, 38, 429-435.

47.Lumbierres, M.;Palomo, J. M.; Kragol,G.; Waldmann ,H.
Solid-phase synthesis of palmitoylated and farnesylated lipopeptides employing the fluoride-labile PTMSEL linker.
Tetrahedron Lett. 2006, 47,2671-2674.

48.Wilson, L.; Palomo, J.M.; Fernández-Lorente, G.; Illanes, A.; Guisán J.M.;. Fernández-Lafuente, R.
Improvement of functional properties of a thermostable lipase from alcaligenes sp. via strong adsorption on hydrophobic supports.
Enzyme Microb. Technol.2006,38,975-980.

49.Torres, R.; Ortiz, C.; Pessela, B.C.; Palomo, J.M.; Mateo, C.; Guisán, J. M.; Fernández-Lafuente,R.
Improvement of the enantioselectivity of lipase (fraction B) from Candida antarctica via adsorpiton on polyethylenimine-agarose under different experimental conditions.
Enzyme Microb. Technol. 2006, 39, 167-171.

50.Mateo, C.; Palomo, J.M.; Fuentes, M.; Betancor,L.; Grazu, V.; Lopez-Gallego, F.; Pessela, B.C.; Hidalgo, A.; Fernández-Lorente,G.; Fernández-Lafuente R.;. Guisán, J. M.
Glyoxyl-agarose:a fully inert hydrophilic support for immobilization and high stabilization of proteins.
Enzyme Microb. Technol. 2006, 39,274-280.

51.Wilson, L.; Palomo, J. M.; Fernández-Lorente, G.; Illanes, A.; Guisán, J. M;. Fernández-Lafuente, R.
Effect of lipase-lipase interactions in the activity,stability and specificity of a lipase from Alcaligenes sp.
Enzyme Microb. Technol.2006, 39,259-264

52.Wilson, L.; Fernández-Lorente, G.; Fernández-Lafuente, R.; Illanes, A.; Guisán, J.M.; *Palomo, J. M.
CLEAs of lipases and poly-ionic polymers: a simple way of preparing stable biocatalysts with improved properties.
Enzyme Microb. Technol. 2006, 39,750-755.

53.Segura, R. L.; Betancor, L.; Palomo, J. M.; Hidalgo,A.; Fernández-Lorente, G.;Terreni, M.; Mateo, C.; Cortés, A.; Fernández-Lafuente,R.;Guisán,J.M.
Purification and identification of the enzyme responsible of most esterasic activity in commercial porcine pancreatic lipase.
Enzyme Microb. Technol. 2006, 39, 817-823

54.de Araújo A.D.; Palomo, J. M.; Cramer, J.; Seizt, O.; Alexandrov, K.; Waldmann H.
Diels-Alder Ligation and Immobilization of Peptides and Proteins.
Chem. Eur. J. 2006. 12, 6095-6109.

55. Fuentes, M.; Pessela, B.C.C.; Mateo, C.; Palomo, Jose M.; Batalla,P.; Fernández-Lafuente, R.; Guisán, Jose M.
Adsorption behaviour of bovine serum albumin on lowly activated anionic exchangers suggests a New strategy for solid–phase proteomics.
Biomacromolecules. 2006, 7,1357-1361.

56.Fernández-Lorente, G.;Palomo, Jose M.; Mateo,C.; Munilla, R.; Cabrera, Z.; Guisán, J.M.; Fernández-Lafuente, R.
Glutaraldehyde crosslinking in the presence of detergents of lipases adsorbed on aminated supports; Improving lipases performance.
Biomacromolecules. 2006, 7,2610-2615.

32.Palomo,J.M.;Segura,R.L.;Mateo,C.;Terreni,M.;Guisan,Jose M.; Fernandez-Lafuente, R.
Synthesis of enantiomerically pure glycidol via a fully enantioselective lipase-catalyzed resolution.
Tetrahedron:Asymmetry.2005,16,869 –874.

33.Palomo, J. M.;Ortiz,C.; Fernandez-Lorente,G.; Fuentes,M.; Guisan, Jose M.; Fernandez-Lafuente,R.
Lipase–lipase interactions as a new tool to immobilize and modulate the lipase properties
Enzyme Microb. Technol.2005,37,456-462.

34.Mateo, C.; Abian, O.; Bernedo, M.; Cuenca, E.; Fuentes, M.; Fernández-Lorente, G.; Palomo, J. M.; Grazu, V.; Pessela, B.C.C.;
Giacomini, C.; Irazoqui, G.; Villarino, A.; Ovsejevi, K.; Batista.-Viera,F.; Fernández-Lafuente,R.; Guisán, J.M.
Special mechanism of immobilization of proteins in glyoxyl supports
Enzyme Microb. Technol.2005,36,447–454.

35.Fernández-Lorente, G.;Ortiz,C.; Segura,R.L.; Fernández-Lafuente, R.; Guisán, Jose M.; *Palomo, J. M.
Purification of different lipases from Aspergillus niger using a highly selective adsorption on hydrophobic supports
Biotechnol Bioeng. 2005,92,773-779.

36.*Palomo, J.M.; Guisán, J.M.; Fernández Lafuente,R.
Solid-phase lipase engineering: a new tool in combinatorial biocatalysis.
ChemInform, 2005, 36 (28)

37.Mateo, C.; Abian, O.; Grazu,V.; Fernández-Lorente, G.; Palomo, J. M.; Fuentes, M.; Segura, R.L.; Montes, T.; Lopez-Gallego, F.;
Wilson, L.; Torres, R.; Guisán, J.M:; Fernández-Lafuente, R.
Recent advances in enzymatic industrial synthesis of semi-synthetic b-lactam antibiotics.
Med. Chem. Rev.2005, 2,207-218.

38.Palomo, J.M.; Fernández-Lorente, G.; Ortiz, C.; Segura, R.L.; Mateo, C.; Fuentes, M.; Hermoso, J.; Fernández-Lafuente, R; Guisán, J.M.
Conformational engineering of lipases via directed immobilization improving the resolution of chiral drugs.
Med. Chem. Rev.2005, 2,369-378.

39.Lumbierres, M., Palomo, J. M.; Kragol, G.; Roehrs, S.; Müller, O.; Waldmann, H.
Solid-phase synthesis of lipopeptides
Chem. Eur. J. 2005,11,7405-7415.

40.Fuentes, M.; Palomo, J.M.; Mateo,C.; Venteo, A.; Saenz, A.; Fernández-Lafuente R.; Guisán, Jose M.
Optimization of the modification of carrier proteins with aminated haptens
J. Immunol. Meth. 2005, 307, 144-149.

20.Palomo, J. M.;Segura, R.L.; Mateo,C.; Guisán,J.M.; Fernández-Lafuente,R.
Improving the activity of lipases from Thermus sp. at moderate temperatures for biotechnology applications.
Biomacromolecules. 2004, 5,249-254.                       

21. Fernández-Lorente, G.; Palomo, J. M.; Mateo, C.; Guisán, J. M.; Fernández-Lafuente,R.
Resolution of paroxetine precursor using different lipases.Influence of the reaction conditions on the enantioselectivity of lipases.
Enzyme Microb. Technol. 2004,34,264-269.

22. Palomo, J.M.; Segura, R.L.; Fernández-Lorente, G.; Guisán, J. M.; Fernández-Lafuente,R.
Enzymatic Resolution of (±)-glycidyl butyrate in aqueous media. Strong modulation of the properties of the lipase from Rhizopus oryzae via immobilization techniques.
Tetrahedron: Asymmetry.2004,15,1157-1161.

23. Mateo, C.; Palomo, J. M.; Van Langen, L.M.; Van Rantwijk, F.; Sheldon, R.
A new mild cross-linking methodology to prepare cross-linked enzyme aggregates.
Biotechnol. Bieng.2004, 86,273-276.

24. Palomo, J. M.; Segura, R. L.; Fernández-Lorente, G.; Pernas, M.; Rua, M.L.;Guisán, J.M.; Fernández-Lafuente,R.
Purification, Immobilization and Stabilization of a lipase from Bacillus thermocatenulatus by Interfacial Adsorption on Hydrophobic Supports.
Biotechnol Prog. 2004, 20,630-635.

25. Abian, O.; Mateo, C.; Palomo, J. M.; Fernández-Lorente, G.; Guisán, J.M.; Fernández-Lafuente,R.
Modulation of penicillin G Acylase properties by the acyl donor in enantioselectivity síntesis of phenylacetamides in the presence of high organic co-solvent concentration.
Biotechnol Prog.2004,30,984-988.

26. Segura, R.L.; Palomo, J. M.; Mateo,C.; Terreni, M.; Guisán, J. M.; Fernández-Lafuente,R.
Evaluation of three different fractions of lipases contained in pancreatic pig live extracts as enantioselective biocatalysts.
Biotechnol Prog. 2004,20,825-829.

27. Palomo, J. M; Ortiz, C.; Fuentes, M.; Fernández-Lorente,G.; Guisán, J.M; Fernández-Lafuente,R.
Use of immobilized lipases for lipase purification via specific lipase–lipase interactions.
J.Cromatogr.A.2004, 1038, 267-273.

28. Fuentes, M.; Pessela, B.C.C.; Maquiese, J.V; Ortiz, C.; Segura, R.L.;Palomo, J. M.; Abian, O; Guisán,J.M.; Mateo,C.; Fernández- Lafuente,R.
Reversible and Strong Immobilization of Proteins by Ionic Exchange on Supports Coated with Sulfate-Dextran
Biotech Prog.2004, 20, 1134-1139.

29. Kragol, G.; Lumbierres, M.; Palomo, J. M.; Waldmann,H.
Solid-Phase Synthesis of Lipidated Peptides.
Angew. Chem. Int Ed. 2004,43, 5839-5842.

30. *Palomo, J.M ; Guisán, J. M.; Fernández Lafuente, R.
Solid-phase lipase engineering:a new tool in combinatorial biocatalysis.
Pharma Chem,2004, 3, 42-45.

11. Palomo, J.M.; Peña, M.; Fernández-Lorente, G.; Mateo, C.; Pisabarro, A.G.; Fernández-Lafuente, R.;Ramirez. L.; Guisán, J.M.
Solid Phase Handling of Hydrophobins: Immobilized Hydrophobins as a New Tool to Study Lipases.
Biomacromolecules.2003,4,204-210.

12. Palomo, J.M.; Muñoz, G.; Fernández-Lorente, G.; Mateo, C.; Fuentes, M.; Guisán J.M.; Fernández-Lafuente, R.
Modulation of Mucor miehei lipase properties via directed immobilization on different hetero-functional epoxy resins.Hydrolytic resolution of (R,S)-2-butyroyl-2-phenylacetic acid.
J. Mol. Cat B: Enzymatic.2003, 21,201-210.

13. Palomo, J. M.; Fuentes, M.; Fernández-Lorente, G.; Mateo,C.; Guisán, J.M.; Fernández-Lafuente,R.
General trend of Lipase to auto-assemble giving bi-molecular aggregates greatly modifies the enzyme functionality.
Biomacromolecules.2003,4,1-6.

14. Palomo, J. M.; Mateo,C.; Fernández-Lorente,G.; Solares , L.F.; Diaz, M.;. Sánchez, V.M.; Bayod,M.;
Gotor, V.; Fernández-Lafuente ,R.;Guisán , J.M.
Chiral Resolution of (R,S)-5-substituted-6-(5-chloropyridin-2-yl)-7-oxo-5,6-dihydropyrrolo[3,4b] pyrazine derivatives-precursors of (S)-(+)-Zopiclone catalyzed by immobilized Candida antarctica B lipase in aqueous media.
Tetrahedron: Asymmetry. 2003, 14, 429-438.

15. Fernández-Lorente, G.; Palomo, J. M.; Fuentes, M.; Mateo,C.; Guisán, J.M; Fernández-Lafuente, R.
Self-assembly of Pseudomonas fluorescens lipase into bimolecular aggregates dramatically affects functional properties.
Biotechnol.Bieng. 2003,82,232-237.

16. Mateo, C.; Torres,R.; Fernández-Lorente,G.; Ortiz, C.; Fuentes, M.; Hidalgo, A.; López-Gallego,F.;
Abian,O.; Palomo, J. M.; Betancor, L.; Pessela, B.C.C.; Guisán, J.M.; Fernández-Lafuente,R.
Epoxy-amino groups: a new tool for improved immobilization of proteins by the epoxy method.
Biomacromolecules. 2003,4,772-777.

17. Torres,R.; Mateo,C.; Ortiz,C.; Fuentes, M.; Palomo, J. M.; Guisán,J.M.; Fernández-Lafuente,R.
A novel heterofunctional epoxy-amino sepabeads for a new enzyme immobilization protocol: immobilization-stabilization of beta-galactosidase from A. oryzae.
Biotechnol.Prog. 2003, 19,1056-1060.

18. Fernández-Lorente, G.; Palomo, J. M.; Cocca, J.; Mateo, C.; Fernández-Lafuente, R.;
Moro, P.;Terreni ,M.; Guisán, J. M.
Regio-selective deprotection of peracetylated sugars via lipase hydrolysis.
Tetrahedron. 2003, 59,5705-5711.

19. Palomo, J. M.; Fernández-Lorente, G.; Rúa, M.L.; Guisán,J.M.; Fernández-Lafuente,R.
Evaluation of the lipase from Bacillus thermocatenulatus as an enantioselective biocatalyst.
Tetrahedron: Asymmetry.2003, 14,3679-3687.

3. Palomo, Jose M.; Fernández-Lorente, G.; Mateo,C.; Ortiz,C.; Fernández-Lafuente,R.; Guisán J,M.
Modulation of the enantioselectivity of lipases via controlled immobilization and medium engineering Hydrolytic resolution of Mandelic acid esters.
Enzyme Microb. Technol. 2002,31,775-783.

4. Palomo, Jose M.; Fernández-Lorente, G.; Mateo, C.; Fuentes, M.; Fernández-Lafuente, R.; Guisán, JM.
Modulation of the enantioselectivity of Candida antarctica B lipase via conformational engineering: kinetic resolution of (±)-alfa-hydroxy-phenylacetic acid derivatives.
Tetrahedron: Asymmetry. 2002,13,1337-1345.

5. Palomo, Jose M.; Fernández-Lorente, G.; Mateo,C.; Fuentes,M.; Guisán ,J.M.; Fernández-Lafuente, R..
Enzymatic production of (3S,4R)-4-(4´-fluorophenyl)-6-oxo-piperidin-3- carboxylic acid using a commercial preparation of lipase A from Candida antarctica: the role of a contaminant esterase.
Tetrahedron:Asymmetry.2002, 13,2653-2659.

6. Palomo, Jose M.; Fernández-Lorente,G.; Mateo,C.; Fernández-Lafuente ,R.; Guisán, J. M.
Enzymatic resolution of (±)-trans-4-(4´-fluorophenyl)-6-oxopiperidin-3-ethyl carboxilate, intermediate in synthesis of (-)-Paroxetine.
Tetrahedron: Asymmetry. 2002,13,2375-2381.

7. Palomo, Jose M.; Muñoz,G.; Fernández-Lorente, G.; Mateo, C.; Fernández-Lafuente,R.; Guisán, J.M.
Interfacial adsorption of lipases on very hydrophobic support (Octadecyl-Sepabeads): Immobilization, hyperactivation and stabilization of the open form of lipases.
J. Mol. Cat B: Enzymatic. 2002,19-20,279-286.

8. Abian, O.; Wilson, L.; Mateo, C.; Fernández-Lorente, G.; Palomo, J.M.; Fuentes,M.; Fernández-Lafuente, R.;
Guisán, J.M.;Re, D.; Tam, A.; Daminatti, M.
Preparation of artificial hyper-hydrophilic micro-environments (polymeric salts) surrounding immobilized enzyme molecules. New enzyme derivatives to be used in any reaction medium.
J. Mol. Cat B: Enzymatic. 2002,19-20,295-303.

9. Torres,R.; Mateo,C.; Fuentes, M.; Palomo, J. M.; Ortiz,C.; Fernández-Lafuente ,R.;
Guisán, J.M.; Tam,A.; Daminati, M.
Reversible Immobilization of Invertase on Sepabeads Coated with Polyethyleneimine: Optimization of the Biocatalyst’s Stability.
Biotechnol. Prog.2002, 18,1221-1226.

10. Fernández-Lafuente, R.; Mateo,C.; Abian, O.; Fernández-Lorente, G.; Palomo, J. M.; Fuentes,M.; Guisán, J. M.
Industrial synthesis of semi-synthetic beta-lactam antibiotics: recent developments in enzyme biocatalysis for improved and more sustainable proceses.
Curr. Med. Chem.: Anti-infective agents. 2002,1,375-387.