Switch off/switch on of a cysteinyl protease as a way to preserve the active catalytic group by modification with a reversible covalent thiol modifier: Immobilization of ficin on vinyl-sulfone activated supports

Highlights, Publications 2022

Switch off/switch on of a cysteinyl protease as a way to preserve the active catalytic group by modification with a reversible covalent thiol modifier: Immobilization of ficin on vinyl-sulfone activated supports

Authors: Morellon-Sterling, R; Bolivar, JM; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 220. page: 0141-8130.
Date: nov-01. 2022.
Doi: 10.1016/j.ijbiomac.2022.08.155.

Abstract:
The immobilization of ficin (a cysteinyl proteases) on vinyl sulfone agarose produced its almost full inactivation. It was observed that the incubation of the free and immobilized enzyme in beta-mercaptoethanol produced a 20 % of enzyme activity recovery, suggesting that [read more]

Arturo Pérez2023-01-15T12:33:26+01:00Categories: Highlights, Publications 2022|Tags: Applied; Polymer Science, Biochemistry & Molecular Biology; Chemistry|

Lipase immobilization via cross-linked enzyme aggregates: Problems and prospects – A review

Highlights, Publications 2022

Lipase immobilization via cross-linked enzyme aggregates: Problems and prospects – A review

Authors: Sampaio, CS; Angelotti, JAF; Fernandez-Lafuente, R; Hirata, DB

Review.
Int. J. Biol. Macromol.. vol: 215. page: 0141-8130.
Date: AUG 31. 2022.
Doi: 10.1016/j.ijbiomac.2022.06.139.

Abstract:
In this review we have focused on the preparation of cross-linked enzyme aggregates (CLEAs) from lipases, as these are among the most used enzyme in bioprocesses. This immobilization method is considered very attractive due to preparation simplicity, [read more]

Arturo Pérez2022-10-07T13:52:46+02:00Categories: Highlights, Publications 2022|Tags: Applied; Polymer Science, Biochemistry & Molecular Biology; Chemistry|

The combination of covalent and ionic exchange immobilizations enables the coimmobilization on vinyl sulfone activated supports and the reuse of the most stable immobilized enzyme

Highlights, Publications 2022

The combination of covalent and ionic exchange immobilizations enables the coimmobilization on vinyl sulfone activated supports and the reuse of the most stable immobilized enzyme

Authors: Arana-Pena, S; Carballares, D; Morellon-Sterling, R; Rocha-Martin, J; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 199. page: 0141-8130.
Date: feb-28. 2022.
Doi: 10.1016/j.ijbiomac.2021.12.148.

Abstract:
The coimmobilization of lipases from Rhizomucor miehei (RML) and Candida antarctica (CALB) has been intended using agarose beads activated with divinyl sulfone. CALB could be immobilized on this support, while RML was not. However, RML was [read more]

Arturo Pérez2022-10-07T13:28:45+02:00Categories: Highlights, Publications 2022|Tags: Applied; Polymer Science, Biochemistry & Molecular Biology; Chemistry|

beta-Galactosidase from Kluyveromyces lactis: Characterization, production, immobilization and applications- A review

Authors: de Albuquerque, TLD; de Sousa, M; Silva, NCGE; Girao Neto, CAC; Gonsalves, LRB; Fernandez-Lafuente, R; Rocha, MVP

Review.
Int. J. Biol. Macromol.. vol: 191. page: 0141-8130.
Date: nov-30. 2021.
Doi: 10.1016/j.ijbiomac.2021.09.133.

Abstract:
A review on the enzyme beta-galactosidase from Kluyveromyces lactis is presented, from the perspective of its structure and mechanisms of action, the main catalyzed reactions, the key factors influencing [read more]

Arturo Pérez2021-11-30T00:00:00+01:00Categories: Publications 2021|Tags: Applied; Polymer Science, Biochemistry & Molecular Biology; Chemistry|

Immobilization of papain: A review

Authors: Tacias-Pascacio, VG; Morellon-Sterling, R; Castaneda-Valbuena, D; Berenguer-Murcia, A; Kamli, MR; Tavano, O; Fernandez-Lafuente, R

Review.
Int. J. Biol. Macromol.. vol: 188. page: 0141-8130.
Date: oct-01. 2021.
Doi: 10.1016/j.ijbiomac.2021.08.016.

Abstract:
Papain is a cysteine protease from papaya, with many applications due to its broad specificity. This paper reviews for first time the immobilization of papain on different supports (organic, inorganic or hybrid [read more]

Arturo Pérez2021-10-01T00:00:00+02:00Categories: Publications 2021|Tags: Applied; Polymer Science, Biochemistry & Molecular Biology; Chemistry|

The β-galactosidase immobilization protocol determines its performance as catalysts in the kinetically controlled synthesis of lactulose

Publications 2021

The β-galactosidase immobilization protocol determines its performance as catalysts in the kinetically controlled synthesis of lactulose

Authors: Neto, CACG; Silva, NCGE; Costa, TD; de Albuquerque, TL; Goncalves, LRB; Fernandez-Lafuente, R; Rocha, MVP

Article.
Int. J. Biol. Macromol.. vol: 176. page: 0141-8130.
Date: APR 15. 2021.
Doi: 10.1016/j.ijbiomac.2021.02.078.

Abstract:
In this paper, 3 different biocatalysts of beta-galactosidase from Kluyveromyces lactis have been prepared by immobilization in chitosan activated with glutaraldehyde (Chi_Glu_Gal), glyoxyl agarose (Aga_Gly_Gal) and agarose coated with polyethylenimine [read more]

Arturo Pérez2021-07-01T20:16:16+02:00Categories: Publications 2021|Tags: Applied; Polymer Science, Biochemistry & Molecular Biology; Chemistry|

Ficin: A protease extract with relevance in biotechnology and biocatalysis

Authors: Morellon-Sterling, R; El-Siar, H; Tavano, OL; Berenguer-Murcia, A; Fernandez-Lafuente, R

Review.
Int. J. Biol. Macromol.. vol: 162. page: 0141-8130.
Date: nov-01. 2020.
Doi: 10.1016/j.ijbiomac.2020.06.144.

Abstract:
Due to the problems raised by the use of animal or microbial recombinant proteases, the use of proteases from vegetable origin is becoming increasingly popular.. Among them, sulfidryl proteases have a special interest. Ficin is an [read more]

Arturo Pérez2020-11-01T00:00:00+01:00Categories: Publications 2020|Tags: Applied; Polymer Science, Biochemistry & Molecular Biology; Chemistry|

Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering

Authors: Arana-Pena, S; Rios, NS; Mendez-Sanchez, C; Lokha, Y; Carballares, D; Goncalves, LRB; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 145. page: 0141-8130.
Date: feb-15. 2020.
Doi: 10.1016/j.ijbiomac.2019.10.087.

Abstract:
This paper shows the step by step coimmobilization of up to five different enzymes following two different orders in the coimmobilization to alter the effect of substrate diffusion limitations. The enzymes were [read more]

Arturo Pérez2020-05-04T10:41:56+02:00Categories: Publications 2020|Tags: Applied; Polymer Science, Biochemistry & Molecular Biology; Chemistry|

Use of glyoxyl-agarose immobilized ficin extract in milk coagulation: Unexpected importance of the ficin loading on the biocatalysts

Authors: Siar, E; Morellon-Sterling, R; Zidoune, MN; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 144. page: 0141-8130.
Date: feb-01. 2020.
Doi: 10.1016/j.ijbiomac.2019.12.140.

Abstract:
A protein extract obtained from fig tree (Ficus carica) latex containing ficin activity was immobilized on glyoxyl agarose. Different biocatalyst loadings were used (3, 10, 30 and 85 mg/g). When casein was used as substrate, the expressed activities [read more]

Arturo Pérez2020-05-04T10:42:01+02:00Categories: Publications 2020|Tags: Applied; Polymer Science, Biochemistry & Molecular Biology; Chemistry|

Optimized immobilization of polygalacturonase from Aspergillus niger following different protocols: Improved stability and activity under drastic conditions

Authors: Dal Magro, L; Kornecki, JF; Klein, MP; Rodrigues, RC; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 138. page: 0141-8130.
Date: oct-01. 2019.
Doi: 10.1016/j.ijbiomac.2019.07.092.

Abstract:
Polygalacturonase (PG) from Aspergillus niger was immobilized using glyoxyl, vinylsulfone or glutaraldehyde-activated supports. The use of supports pre-activated with glutaraldehyde presented the best results. The immobilization of PG on glutaraldehyde-supports was studied under different conditions: [read more]

Arturo Pérez2020-04-20T18:18:30+02:00Categories: Publications 2019|Tags: Applied; Polymer Science, Biochemistry & Molecular Biology; Chemistry|

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