Applied; Polymer Science

Authors: de Albuquerque, TLD; de Sousa, M; Silva, NCGE; Girao Neto, CAC; Gonsalves, LRB; Fernandez-Lafuente, R; Rocha, MVP

Review.
Int. J. Biol. Macromol.. vol: 191. page: 0141-8130.
Date: nov-30. 2021.
Doi: 10.1016/j.ijbiomac.2021.09.133.

Abstract:
A review on the enzyme beta-galactosidase from Kluyveromyces lactis is presented, from the perspective of its structure and mechanisms of action, the main catalyzed reactions, the key factors influencing [read more]

Authors: Tacias-Pascacio, VG; Morellon-Sterling, R; Castaneda-Valbuena, D; Berenguer-Murcia, A; Kamli, MR; Tavano, O; Fernandez-Lafuente, R

Review.
Int. J. Biol. Macromol.. vol: 188. page: 0141-8130.
Date: oct-01. 2021.
Doi: 10.1016/j.ijbiomac.2021.08.016.

Abstract:
Papain is a cysteine protease from papaya, with many applications due to its broad specificity. This paper reviews for first time the immobilization of papain on different supports (organic, inorganic or hybrid [read more]

Authors: Dal Magro, L; Kornecki, JF; Klein, MP; Rodrigues, RC; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 138. page: 0141-8130.
Date: oct-01. 2019.
Doi: 10.1016/j.ijbiomac.2019.07.092.

Abstract:
Polygalacturonase (PG) from Aspergillus niger was immobilized using glyoxyl, vinylsulfone or glutaraldehyde-activated supports. The use of supports pre-activated with glutaraldehyde presented the best results. The immobilization of PG on glutaraldehyde-supports was studied under different conditions: [read more]

Authors: Dal Magro, L; Kornecki, JF; Klein, MP; Rodrigues, RC; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 138. page: 0141-8130.
Date: oct-01. 2019.
Doi: 10.1016/j.ijbiomac.2019.07.092.

Abstract:
Polygalacturonase (PG) from Aspergillus niger was immobilized using glyoxyl, vinylsulfone or glutaraldehyde-activated supports. The use of supports pre-activated with glutaraldehyde presented the best results. The immobilization of PG on glutaraldehyde-supports was studied under different conditions: [read more]

Authors: Siar, E; Morellon-Sterling, R; Zidoune, MN; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 133. page: 0141-8130.
Date: jul-15. 2019.
Doi: 10.1016/j.ijbiomac.2019.04.123.

Abstract:
Ficin extract has been aminated using ethylenediamine and carbodiimide to transform all exposed carboxylic groups into amino groups, retaining around 80% of activity versus benzoyl-D,L-argininep-nitroanilide hydrochloride (BANA) and 90% versus casein. This aminated enzyme was then immobilized on [read more]

Authors: Arana-Pena, S; Mendez-Sanchez, C; Rios, NS; Ortiz, C; Goncalves, LRB; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 131. page: 0141-8130.
Date: jun-15. 2019.
Doi: 10.1016/j.ijbiomac.2019.03.163.

Abstract:
Lipase B from Candida antarctica (CALB), lipase from Rhizomucor miehei (RML) and phospholipase Lecitase Ultra (LEU) were immobilized via interfacial activation and their stabilities were compared. Immobilized CALB was much more stable than immobilized [read more]