Switch off/switch on of a cysteinyl protease as a way to preserve the active catalytic group by modification with a reversible covalent thiol modifier: Immobilization of ficin on vinyl-sulfone activated supports

Highlights, Publications 2022

Switch off/switch on of a cysteinyl protease as a way to preserve the active catalytic group by modification with a reversible covalent thiol modifier: Immobilization of ficin on vinyl-sulfone activated supports

Authors: Morellon-Sterling, R; Bolivar, JM; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 220. page: 0141-8130.
Date: nov-01. 2022.
Doi: 10.1016/j.ijbiomac.2022.08.155.

Abstract:
The immobilization of ficin (a cysteinyl proteases) on vinyl sulfone agarose produced its almost full inactivation. It was observed that the incubation of the free and immobilized enzyme in beta-mercaptoethanol produced a 20 % of enzyme activity recovery, suggesting that [read more]

Arturo Pérez2023-01-15T12:33:26+01:00Categories: Highlights, Publications 2022|Tags: Applied; Polymer Science, Biochemistry & Molecular Biology; Chemistry|

Lipase immobilization via cross-linked enzyme aggregates: Problems and prospects – A review

Highlights, Publications 2022

Lipase immobilization via cross-linked enzyme aggregates: Problems and prospects – A review

Authors: Sampaio, CS; Angelotti, JAF; Fernandez-Lafuente, R; Hirata, DB

Review.
Int. J. Biol. Macromol.. vol: 215. page: 0141-8130.
Date: AUG 31. 2022.
Doi: 10.1016/j.ijbiomac.2022.06.139.

Abstract:
In this review we have focused on the preparation of cross-linked enzyme aggregates (CLEAs) from lipases, as these are among the most used enzyme in bioprocesses. This immobilization method is considered very attractive due to preparation simplicity, [read more]

Arturo Pérez2022-10-07T13:52:46+02:00Categories: Highlights, Publications 2022|Tags: Applied; Polymer Science, Biochemistry & Molecular Biology; Chemistry|

Unraveling Membrane Perturbations Caused by the Bacterial Riboregulator Hfq

Authors: Turbant, F; Waeytens, J; Campidelli, C; Bombled, M; Martinez, D; Grelard, A; Habenstein, B; Raussens, V; Velez, M; Wien, F; Arluison, V

Article.
Int. J. Mol. Sci.. vol: 23. page: .
Date: AUG. 2022.
Doi: 10.3390/ijms23158739.

Abstract:
Hfq is a pleiotropic regulator that mediates several aspects of bacterial RNA metabolism. The protein notably regulates translation efficiency and RNA decay in Gram-negative [read more]

Arturo Pérez2022-08-14T00:00:00+02:00Categories: Publications 2022|Tags: Biochemistry & Molecular Biology; Chemistry, Multidisciplinary|

The combination of covalent and ionic exchange immobilizations enables the coimmobilization on vinyl sulfone activated supports and the reuse of the most stable immobilized enzyme

Highlights, Publications 2022

The combination of covalent and ionic exchange immobilizations enables the coimmobilization on vinyl sulfone activated supports and the reuse of the most stable immobilized enzyme

Authors: Arana-Pena, S; Carballares, D; Morellon-Sterling, R; Rocha-Martin, J; Fernandez-Lafuente, R

Article.
Int. J. Biol. Macromol.. vol: 199. page: 0141-8130.
Date: feb-28. 2022.
Doi: 10.1016/j.ijbiomac.2021.12.148.

Abstract:
The coimmobilization of lipases from Rhizomucor miehei (RML) and Candida antarctica (CALB) has been intended using agarose beads activated with divinyl sulfone. CALB could be immobilized on this support, while RML was not. However, RML was [read more]

Arturo Pérez2022-10-07T13:28:45+02:00Categories: Highlights, Publications 2022|Tags: Applied; Polymer Science, Biochemistry & Molecular Biology; Chemistry|

Production and Digestibility Studies of beta-Galactosyl Xylitol Derivatives Using Heterogeneous Catalysts of LacA beta-Galactosidase from Lactobacillus Plantarum WCFS1

Authors: Rosado, E; Delgado-Fernandez, P; de las Rivas, B; Munoz, R; Moreno, FJ; Corzo, N; Mateo, C

Article.
Molecules. vol: 27. page: .
Date: FEB. 2022.
Doi: 10.3390/molecules27041235.

Abstract:
The synthesis of beta-galactosyl xylitol derivatives using immobilized LacA beta-galactosidase from Lactobacillus plantarum WCFS1 is presented. These compounds have the potential to replace traditional sugars by their properties as sweetener and taking the [read more]

Arturo Pérez2022-05-05T18:15:22+02:00Categories: Publications 2022|Tags: Biochemistry & Molecular Biology; Chemistry, Multidisciplinary|

beta-Galactosidase from Kluyveromyces lactis: Characterization, production, immobilization and applications- A review

Authors: de Albuquerque, TLD; de Sousa, M; Silva, NCGE; Girao Neto, CAC; Gonsalves, LRB; Fernandez-Lafuente, R; Rocha, MVP

Review.
Int. J. Biol. Macromol.. vol: 191. page: 0141-8130.
Date: nov-30. 2021.
Doi: 10.1016/j.ijbiomac.2021.09.133.

Abstract:
A review on the enzyme beta-galactosidase from Kluyveromyces lactis is presented, from the perspective of its structure and mechanisms of action, the main catalyzed reactions, the key factors influencing [read more]

Arturo Pérez2021-11-30T00:00:00+01:00Categories: Publications 2021|Tags: Applied; Polymer Science, Biochemistry & Molecular Biology; Chemistry|

Immobilization of papain: A review

Authors: Tacias-Pascacio, VG; Morellon-Sterling, R; Castaneda-Valbuena, D; Berenguer-Murcia, A; Kamli, MR; Tavano, O; Fernandez-Lafuente, R

Review.
Int. J. Biol. Macromol.. vol: 188. page: 0141-8130.
Date: oct-01. 2021.
Doi: 10.1016/j.ijbiomac.2021.08.016.

Abstract:
Papain is a cysteine protease from papaya, with many applications due to its broad specificity. This paper reviews for first time the immobilization of papain on different supports (organic, inorganic or hybrid [read more]

Arturo Pérez2021-10-01T00:00:00+02:00Categories: Publications 2021|Tags: Applied; Polymer Science, Biochemistry & Molecular Biology; Chemistry|

Sunlight-Operated TiO2-Based Photocatalysts

Authors: Barba-Nieto, I; Caudillo-Flores, U; Fernandez-Garcia, M; Kubacka, A

Review.
Molecules. vol: 25. page: .
Date: SEP. 2020.
Doi: 10.3390/molecules25174008.

Abstract:
Photo-catalysis is a research field with broad applications in terms of potential technological applications related to energy production and managing, environmental protection, and chemical synthesis fields. A global goal, common to all of these fields, is to generate photo-catalytic materials able [read more]

Arturo Pérez2021-09-02T00:00:00+02:00Categories: Publications 2021|Tags: Biochemistry & Molecular Biology; Chemistry, Multidisciplinary|

In Search of Effective Anticancer Agents-Novel Sugar Esters Based on Polyhydroxyalkanoate Monomers

Authors: Snoch, W; Wnuk, D; Witko, T; Staron, J; Bojarski, AJ; Jarek, E; Plou, FJ; Guzik, M

Article.
Int. J. Mol. Sci.. vol: 22. page: .
Date: JUL. 2021.
Doi: 10.3390/ijms22137238.

Abstract:
Cancer is one of the deadliest illness globally. Searching for new solutions in cancer treatments is essential because commonly used mixed, targeted and personalized therapies are sometimes not sufficient or [read more]

Arturo Pérez2021-07-29T00:00:00+02:00Categories: Publications 2021|Tags: Biochemistry & Molecular Biology; Chemistry, Multidisciplinary|

Preparation, Characterization, and Activity of Pd/PSS-Modified Membranes in the Low Temperature Dry Reforming of Methane with and without Addition of Extra Steam

Authors: Mateos-Pedrero, C; Soria, MA; Guerrero-Ruiz, A; Rodriguez-Ramos, I

Article.
Membranes. vol: 11. page: .
Date: JUL. 2021.
Doi: 10.3390/membranes11070518.

Abstract:
The external surface of a commercial porous stainless steel (PSS) was modified by either oxidation in air at varying temperatures (600, 700, and 800 degrees C) or coating with different oxides (SiO2, Al2O3, and ZrO2). Among them, PSS-ZrO2 appears as the [read more]

Arturo Pérez2021-07-29T00:00:00+02:00Categories: Publications 2021|Tags: Biochemistry & Molecular Biology; Chemistry, Chemical; Materials Science, Multidisciplinary; Polymer Science, Physical; Engineering|

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