Viña González, Javier

Profile

Contact

email: javier.v@csic.es
teléfono: 915855479/915854822
Name Javier
Surnames Viña González
E-mail E-mail: javier.v@csic.es
Department Biocatálisis
Research Group

Evolución Dirigida de Enzimas
Biography

Biography:

The focus of Javier Viña research is to develop an evolutionary platform for the aryl-alcohol oxidase (AAO) from Pleurotus eryngii in order to obtain functional expression in yeasts and, as well, to design a secondary benzyl-alcohol oxidase and explore the enzymatic conversion of furfural derivatives.

Javier obtained a B.Sc. Biology from the Universidad de Oviedo in 2012 and in 2013 he took master studies on Biotechnology, at Universidad Autónoma de Madrid, when he also joined the laboratory of PhD Miguel Alcalde to carry out his master thesis on “Chimeric signal peptides for functional expression of aryl-alcohol oxidase in Saccharomyces cerevisiae”.

Once he finished, he continued on the laboratory of Miguel Alcalde, working on the development of biotechnological platforms for aryl-alcohol oxidases by directed evolution, until he achieved the PhD degree on 2019.
Besides, he is one of the cofounders of the startup EvoEnzyme, derived from the laboratory of Miguel Alcalde to translate the achievements on enzymatic directed evolution to the society.
Publications

Publications:

• Viña-Gonzalez, J., Jimenez-Lalana, D., Sancho, F., Serrano, A., Martinez, A.T., Guallar, V., Alcalde, M. Structure-Guided evolution of aryl alcohol oxidase from Pleurotus eryngii for the selective oxidation of secondary benzyl alcohols. Advanced Synthesis and Catalysis. 2019. DOI: 10.1002/adsc.201900134.

• Vicente, A.I., Viña-Gonzalez, J., Mateljak, I., Monza, E., Lucas, F., Guallar, V., Alcalde, M. Enhancing thermostability by modifying flexible surface loops in an evolved high-redox potential laccase. AIChE Journal. 2019. DOI: 10.1002/aic.16747.
• Serrano, A., Sancho, F., Viña-González, J., Carro, J., Alcalde, M., Guallar, V., Martínez, A.T. Switching the substrate preference of fungal aryl-alcohol oxidase: Towards stereoselective oxidation of secondary benzyl alcohols. Catalysis Science and Technology. 2019. DOI: 10.1039/c8cy02447b.

• Viña-Gonzalez, J., Elbl, K., Ponte, X., Valero, F.and Alcalde, M. Functional expression of aryl-alcohol oxidase in Saccharomyces cerevisiae and Pichia pastoris by directed evolution. Biotechnology & Bioengineering. In press. 2018. https://doi.org/10.1002/bit.26585.

• Vicente, AI., Viña-Gonzalez, J., Santos-Moriano, P., Marquez-Alvarez, C., Ballesteros, AO., and Alcalde, M. Synthesis of C-N heteropolymeric dyes using an evolved alkaline fungal laccase secreted by Saccharomyces cerevisiae. Journal of Molecular Catalysis B: Enzymatic. 2016.

• Molina-Espeja, P., Viña-Gonzalez, J., Gomez, B.J., Martin-Diaz, J., Garcia-Ruiz, E., and Alcalde, M. Beyond the outer limits of nature by directed evolution. Biotechnology Advances. 34: 754-767. 2016. http://doi.org/10.1016/j.biotechadv.2016.03.008.

• Viña-Gonzalez, J., Gonzalez-Perez, D., and Alcalde, M. Directed evolution method in Saccharomyces cerevisiae: Mutant library creation and screening. Journal of Visualized Experiments. 110: e53761.2016. http://doi.org/10.3791/53761.

• Viña-Gonzalez, J., Gonzalez-Perez, D., Ferreira, P., Martinez, A.T., and Alcalde, M. Focused directed evolution of aryl-alcohol oxidase in yeast by using chimeric signal peptides. Applied and Environmental Microbiology. 81: 6451-6462. 2015. http://doi.org/10.1128/AEM.01966-15.

• Viña-Gonzalez, J., Gonzalez-Perez, D., Martinez, A.T., and Alcalde, M. “Chimeric signal peptides for the functional expression of aryl alcohol oxidase in Saccharomyces cerevisiae”. Título del libro: 13th European Workshop on Lignocellulosics and Pulp (EWLP-2014) Proceedings. IBSN: 978-84-616-9842-4.