Description
The Applied Biocatalysis Group was started 40 years ago by Dr. Antonio Ballesteros, and currently leaded by Dr. Francisco J. Plou. The group studies the application of enzymes at different industries, such as biomedics, pharmacologic nutraceutic, pulp industries, functional foods or sustainable chemistry.
Research lines
Personal
Group Staff
Relación de personal del Grupo
Antonio Ballesteros (Prof.)
Lucía Fernández-Arrojo (personal de investigación)
Noa Míguez (Estudiante de doctorado)
José Luis Gonzalez (Estudiante de doctorado)
David Fernández (Estudiante de doctorado)
Fadia Cervantes (Estudiante de doctorado)
Jose Manuel Hernández (Técnico)
Patents
“Enzymatic procedure for the preparation of alfa-glucosylated derivatives of resveratrol with surfactant properties”.
Torres, F.J. Plou, A. Ballesteros, J.L. Parra, F. Comelles, J. Jiménez-Barbero and A. Poveda
Spanish Patent 200930037. PCT/ES2010/070177
“Alginate-based immobilized biocatalyst for the biotransformation of carbohydrates .
Fernández-Arrojo, F.J. Plou, M. Alcalde, A. Ballesteros, M. Fernández-Lobato y P. Gutiérrez-Alonso
Spanish Patent 200930001. PCT/ES2010/070104
“Procedimiento de obtención de una enzima inmovilizada en un soporte renovable derivado de un residuo agroalimentario”.
M.A. Martin-Luengo, M. Yates, F.J. Plou, R. Lozano E. Saez, A.M. Martinez, L. Vega, L. Medina V. Zurdo, M. Ramos, F. Valero, M.D. Benaiges.
Spanish Patent 201330114
Projects
“Novel trends in the food industry for the preparation of bioactive ingredients using last-generation glycosydic enzymes”, GLICO-BIOCAT; Financial Organization: Spanish MINECO; Reference: BIO2016-76601-C3-1-R; Principal Investigators: Francisco J. Plou and Antonio Ballesteros
“Spanish Network on Biotechnology for lignocellulose: Towards the efficient use of biomass as renewable feedstock “, LIGNOCEL; Financial Organization: Ministerio de Economía, Industria y Competitividad; Reference: BIO2017‐90757‐REDT ; Principal Investigator: Susana Camarero (CIB-CSIC)
“International Network for the Search, Evaluation and Application of Enzymes in the Food Industry” , ENZAL; Financial Organization: Comisión Nacional de Investigación Científica y Tecnológica (CONICYT, Chile); Reference: REDI170057; Principal Investigator: Carlos Vera (Universidad de Santiago de Chile)
Publications
Alonso, G. Santiago, I. Cea-Rama, L. Fernandez-Lopez, C. Coscolín, J. Modregger, A. K. Ressmann, M. Martínez-Martínez, H. Marrero, R. Bargiela, M. Pita, J. L. Gonzalez-Alfonso, M. L. Briand, D. Rojo, C. Barbas, F. J. Plou, P. N. Golyshin, P. Shahgaldian, J. Sanz-Aparicio, V. Guallar and M. Ferrer. Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis.Nature Catalysis(2019), doi:10.1038/s41929-019-0394-4
M. Ramirez-Escudero, N. Miguez, M. Gimeno-Perez, A.O. Ballesteros, M. Fernandez-Lobato, F.J. Plou and J. Sanz-Aparicio. Deciphering the molecular specificity of phenolic compounds as inhibitors or glycosyl acceptors of β fructofuranosidase from Xanthophyllomyces dendrorhous. Scientific Reports 9, 17441 (2019), doi:10.1038/s41598-019-53948-y
S. Neifar, F.V. Cervantes, H. BenHlima, A. Bouanane-Darenfed, A.O. Ballesteros, F.J. Plou, S. Bejar. Immobilization of the glucose isomerase from Caldicoprobacter algeriensis on Sepabeads EC-HA and its efficient application in continuous High Fructose Syrup production using packed bed reactor. Food Chemistry (2019), doi: 10.1016/j.foodchem.2019.125710
Garcia-Arellano, J. L. Gonzalez-Alfonso, C. Ubilla, F. Comelles, M. Alcalde, M. Bernabé, J-L Parra, A. O. Ballesteros and F. J. Plou. Production and surfactant properties of tert-butyl α-D-glucopyranosides catalyzed by cyclodextrin Glucanotransferase. Catalysts(2019), doi:10.3390/catal9070575
Santos-Moriano, P. Kidibule, N. Miguez, L. Fernández-Arrojo, A.O. Ballesteros, M. Fernández-Lobato and F. J. Plou. Tailored enzymatic synthesis of chitooligosaccharides with different deacetylation degrees and their anti-inflammatory activity. Catalysts (2019), doi:10.3390/catal9050405
Gomez de Santos, F.V. Cervantes, F. Tieves, F.J. Plou, F. Hollmann, M. Alcalde. Benchmarking of laboratory evolved unspecific peroxygenases for the synthesis of human drug metabolites. Tetrahedron (2019), doi:10.1016/j.tet.2019.02.013 Molina-Espeja, P. Santos-Moriano, E. García-Ruiz, A. Ballesteros, FJ. Plou and M. Alcalde. One-point oriented immobilization of an evolved unspecific peroxygenase. International Journal of Molecular Sciences(2019), doi:10.3390/ijms20071627
J.L. Gonzalez-Alfonso, P. Peñalver, A.O. Ballesteros, J.C. Morales and F.J. Plou. Effect of a-glucosylation on the stability, antioxidant properties, toxicity and neuroprotective activity of (-)-epigallocatechin gallate. Frontiers in Nutrition (2019), doi:10.3389/fnut.2019.00030
J.L. Gonzalez-Alfonso, L. Casas-Godoy, J. Arrizon, D. Arrieta-Baez, A.O. Ballesteros, G. Sandoval and F.J. Plou. Lipase-catalyzed synthesis of fatty acid esters of trisaccharides. Methods Molecular Biology, 1835:287-296. (2018), doi:10.1007/978-1-4939-8672-9_15
J.L. González-Alfonso, N. Míguez, J. D. Padilla, L. Leemans, A. Poveda, J. Jimenez-Barbero, A. O. Ballesteros, G. Sandoval and F. J. Plou. Optimization of Regioselective Glucosylation of Hesperetin Catalyzed by Cyclodextrin Glucanotransferase. Molecules, 23, 2285, (2018) doi:10.3390/molecules23112885
Míguez, M. Ramírez-Escudero, M. Gimeno-Pérez, A. Poveda, J. Jiménez-Barbero, A. O. Ballesteros, M. Fernández-Lobato, J. Sanz-Aparicio and F. J. Plou. Fructosylation of hydroxytyrosol by the β-fructofuranosidase from Xanthophyllomyces dendrorhous: Insights into the molecular basis of the enzyme specificity. ChemCatChem, (2018)doi:10.1002/cctc.201801171
Santos-Moriano, P.E. Kidibule, E. Alleyne, A.O. Ballesteros, A. Heras, M. Fernandez-Lobato, F.J. Plou. Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis. Process Biochemistry, (2018). doi:10.1016/j.procbio.2018.07.017
J.L. Gonzalez-Alfonso, L. Leemans, A. Poveda, J. Jimenez-Barbero, A.O. Ballesteros and F.J. Plou. Efficient α-glucosylation of epigallocatechin gallate catalyzed by cyclodextrin glucanotransferase from Thermoanaerobacter sp. Journal of Agricultural and Food Chemistry 66, 7402–7408 (2018). doi:10.1021/acs.jafc.8b02143
J.L. González-Alfonso, D. Rodrigo-Frutos, E. Belmonte-Reche, P. Peñalver, A. Poveda, J. Jimenez-Barbero, A.O. Ballesteros, Y. Hirose, J. Polaina, J.C. Morales, M. Fernández-Lobato and F. J. Plou. Enzymatic synthesis of a novel pterostilbene α-glucoside by the combination of cyclodextrin glucanotransferase and amyloglucosidase. Molecules 25;23(6) (2018). doi:10.3390/molecules23061271
Miguez, M. Gimeno-Perez, D. Fernandez-Polo, F.V. Cervantes, A.O. Ballesteros, M. Fernandez-Lobato, M.H. Ribeiro, F.J Plou. Immobilization of the β-fructofuranosidase from Xanthophyllomyces dendrorhous by entrapment in polyvinyl alcohol and its application to neo-fructooligosaccharides production. Catalysts8(5), 201 (2018). doi:10.3390/catal8050201
Gomez-Fernandez, E. Garcia-Ruiz, J. Martin, P. Gomez de Santos, P. Santos-Moriano, F.J. Plou, A. Ballesteros, M. Garcia, M. Rodriguez, V.A.
Thesis
Noa Míguez Rodríguez. “Síntesis de oligosacáridos y glicoconjugados bioactivos empleando enzimas glicosídicas”. 2019. Autonomous University of Madrid (UAM). Supervisor: Francisco J. Plou.
Paloma Santos Moriano. “Biocatalytic processes for the production of bioactive carbohydrates; fructooligosaccharides and chitooligosachharides”. 2017. Autonomous University of Madrid (UAM). Supervisor: Francisco J. Plou.
Paolo Zambelli. “Development of new biocatalytic processes for fructooligosaccharides (FOS) preparation”. University Degli Studi di Milano (Italia). 2014. Supervisors: Francesco E. Molinari and Francisco J. Plou.
Bárbara Rodríguez Colinas. “Enzymatic synthesis, characterization and prebiotic properties of oligosaccharides employed in infant foods”. Autonomous University of Madrid (UAM). 2013. Supervisors: Francisco J. Plou and Antonio Ballesteros.
Diana Maté Mate. “Diseño de lacasas fúngicas activas en sangre mediante evolución dirigida”. 2013. Autonomous University of Madrid (UAM). Supervisor: Miguel Alcalde and Francisco J. Plou.
Nora R. Aimaretti. “Development of a sustainable process for the production of bioethanol from agroindustrial wastes”. UNED. 2011. Supervisors: Juan Carlos Yori and Francisco J. Plou.
Lucía Fernández Arrojo. “Biocatalytic processes for the sustainable production of prebiotic oligosaccharides employing immobilized glycosyltransferases”. Autonomous University of Madrid (UAM). 2009. Supervisors: Francisco J. Plou and Antonio Ballesteros.
Pamela Torres Salas. “Stabilization of bioactive phenolic antioxidants of naturalorigin: Modification of tocopherols and resveratrol by enzymatic acylation and glycosylation”. Autonomous University of Madrid (UAM). 2009. Supervisors: Francisco J. Plou and Antonio Ballesteros.