Optimization of the immobilization of xylanase from Thermomyces lanuginosus to produce xylooligosaccharides in a batch type reactor

Autores: Alagoz, D; Varan, NE; Yildirim, D; Fernandez-Lafuente, R

Article.
Mol. Catal.. vol: 531. page: 2468-8231.
Fecha: OCT. 2022.
Doi: 10.1016/j.mcat.2022.112647.

Resumen:
The xylanase from Thermomyces lanuginosus was immobilized following very different protocols, using 3-aminopropyl (Xyl/CNT-NH2) and carboxylic acid (Xyl/CNT-C) functionalized multi-walled carbon nanotubes or after glutaraldehyde activation of CNT-NH2 (Xyl/CNT-Glu). It was also immobilized on carboxylic acid functionalized multi-walled carbon nanotubes via carbodiimide chemistry (Xyl/CNT-COOH). The free and all the immobilized xylanase derivatives showed maximal catalytic activity at pH 7.5 and at 65 ?. Xyl/CNT-Glu showed 24.0-fold higher thermal stability than the free xylanase at 70 ?. The immobilized xylanase derivatives were used for the production of xylooligosaccarides, the reaction courses and the yields showing a dependence on the immobilization protocol. The highest XOS yield (22.1%) was obtained using Xyl/CNT-Glu after 8 h of reaction at pH 75 and 65 ?..