Immobilization-stabilization of the dimeric D-amino acid oxidase from porcine kidney

Authors: Carballares, D; Fernandez-Lafuente, R; Rocha-Martin, J

Article.
Process Biochem.. vol: 122. page: 1359-5113.
Date: NOV. 2022.
Doi: 10.1016/j.procbio.2022.10.002.

Abstract:
This paper shows the immobilization of the dimeric D-amino acid oxidase (DAAO) following different methodologies. Immobilization on glyoxyl-agarose yielded activity recoveries under 5%, and it was discarded. The anion exchange on aminated supports permits the rapid enzyme immobilization, but its activity decreased [read more]

Arturo Pérez2022-11-09T17:55:17+01:00Categories: Publications 2022|Tags: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical|

Positive effect of glycerol on the stability of immobilized enzymes: Is it a universal fact?

Authors: Braham, SA; Siar, E; Arana-Pena, S; Bavandi, H; Carballares, D; Morellon-Sterling, R; de Andrades, D; Kornecki, JF; Fernandez-Lafuente, R

Article.
Process Biochem.. vol: 102. page: 1359-5113.
Date: MAR. 2021.
Doi: 10.1016/j.procbio.2020.12.015.

Abstract:
In this paper, the effect of glycerol at different concentrations and pH values on the stabilities of 13 immobilized enzymes has been analyzed. Among the enzymes, we have selected [read more]

Arturo Pérez2021-04-08T11:57:40+02:00Categories: Publications 2021|Tags: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical|

Parameters necessary to define an immobilized enzyme preparation

Authors: Boudrant, J; Woodley, JM; Fernandez-Lafuente, R

Review.
Process Biochem.. vol: 90. page: 1359-5113.
Date: MAR. 2020.
Doi: 10.1016/j.procbio.2019.11.026.

Abstract:
Biocatalytic processes continue to find increasing application in industry. Therefore enzyme immobilization has also become of increasing importance as a means of allowing enzyme containment within reactors operating in continuous mode or else separation of enzyme after use in (fed-)batch reactors, as [read more]

Arturo Pérez2020-03-20T00:00:00+01:00Categories: Publications 2020|Tags: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical|

Modulation of Lecitase properties via immobilization on differently activated Immobead-350: Stabilization and inversion of enantiospecificity

Authors: Pinheiro, MP; Monteiro, RRC; Silva, FFM; Lemos, TLG; Fernandez-Lafuente, R; Goncalves, LRB; dos Santos, JCS

Article.
Process Biochem.. vol: 87. page: 1359-5113.
Date: DEC. 2019.
Doi: 10.1016/j.procbio.2019.08.016.

Abstract:
Lecitase Ultra (Leci) was immobilized on Immobead-350 (IB-350) activated with epoxy (EPO), amino-divinylsulfone (DVS) or amino-glutaraldehyde (GLU) groups. Leci-GLU had 27% more activity versus p-nitro-phenyl butyrate (p-NPB) than Leci-EPO, after 3 h of [read more]

Arturo Pérez2020-05-04T10:49:14+02:00Categories: Publications 2020|Tags: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical|

Modulation of Lecitase properties via immobilization on differently activated Immobead-350: Stabilization and inversion of enantiospecificity

Authors: Pinheiro, MP; Monteiro, RRC; Silva, FFM; Lemos, TLG; Fernandez-Lafuente, R; Goncalves, LRB; dos Santos, JCS

Article.
Process Biochem.. vol: 87. page: 1359-5113.
Date: DEC. 2019.
Doi: 10.1016/j.procbio.2019.08.016.

Abstract:
Lecitase Ultra (Leci) was immobilized on Immobead-350 (IB-350) activated with epoxy (EPO), amino-divinylsulfone (DVS) or amino-glutaraldehyde (GLU) groups. Leci-GLU had 27% more activity versus p-nitro-phenyl butyrate (p-NPB) than Leci-EPO, after 3 h of [read more]

Arturo Pérez2020-05-04T12:37:03+02:00Categories: Publications 2019|Tags: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical|

Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis

Authors: Santos-Moriano, P; Kidibule, PE; Alleyne, E; Ballesteros, AO; Heras, A; Fernandez-Lobato, M; Plou, FJ

Article.
Process Biochem.. vol: 73. page: 1359-5113.
Date: OCT. 2018.
Doi: 10.1016/j.procbio.2018.07.017.

Abstract:
Starting from a commercial preparation of Bacillus thuringiensis var. aizawai, widely employed as biopesticide in agriculture, we obtained a chitosanolytic activity by washing the cells with concentrated sodium acetate. The optimum pH and temperature [read more]

Arturo Pérez2020-05-29T10:48:26+02:00Categories: publications2018|Tags: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical|

Immobilization of beta-galactosidase in glutaraldehyde-chitosan and its application to the synthesis of lactulose using cheese whey as feedstock

Authors: de Albuquerque, TL; Gomes, SDL; D’Almeida, AP; Fernandez-Lafuente, R; Goncalves, LRB; Rocha, MVP

Article.
Process Biochem.. vol: 73. page: 1359-5113.
Date: OCT. 2018.
Doi: 10.1016/j.procbio.2018.08.010.

Abstract:
The aim of this work was to establish optimal conditions for the enzymatic production of lactulose by beta-galactosidase from Kluyveromyces lactis using cheese whey as feedstock. Initially, the production of this disaccharide was evaluated using [read more]

Arturo Pérez2020-05-29T10:48:02+02:00Categories: publications2018|Tags: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical|

Solid phase chemical modification of agarose glyoxyl-ficin: Improving activity and stability properties by amination and modification with glutaraldehyde

Authors: Siar, E; Arana-Pena, S; Barbosa, O; Zidoune, MN; Fernandez-Lafuente, R

Article.
Process Biochem.. vol: 73. page: 1359-5113.
Date: OCT. 2018.
Doi: 10.1016/j.procbio.2018.07.013.

Abstract:
Ficin extract immobilized on glyoxyl-agarose was chemically modified using carbodiimide and ethylenediamine (full or partial amination of the carboxylic groups of the enzyme) or glutaraldehyde (modifying primary amino groups) under different conditions. Aminated enzyme altered its activity versus [read more]

Arturo Pérez2020-05-29T10:48:11+02:00Categories: publications2018|Tags: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical|

Covalent immobilization-stabilization of beta-1,4-endoxylanases from Trichoderma reesei: Production of xylooligosaccharides

Authors: de Oliveira, SM; Moreno-Perez, S; Terrasan, CRF; Romero-Fernandez, M; Vieira, MF; Guisan, JM; Rocha-Martin, J

Article.
Process Biochem.. vol: 64. page: 1359-5113.
Date: JAN. 2018.
Doi: 10.1016/j.procbio.2017.09.018.

Abstract:
The production of xylooligosaccharides (XOS) was evaluated using immobilized and stabilized biocatalysts of a commercial enzymatic cocktail, Bioxilanase L PLUS (BIO), which is based on the xylanolytic enzymes produced by Trichoderma reesei. BIO [read more]

Arturo Pérez2020-05-29T10:59:43+02:00Categories: publications2018|Tags: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical|

A new heterofunctional amino-vinyl sulfone support to immobilize enzymes: Application to the stabilization of beta-galactosidase from Aspergillus oryzae

Authors: Zaak, H; Sassi, M; Fernandez-Lafuente, R

Article.
Process Biochem.. vol: 64. page: 1359-5113.
Date: JAN. 2018.
Doi: 10.1016/j.procbio.2017.09.020.

Abstract:
The paper shows the preparation of a new heterofunctional agarose support: amino-vinylsulfone. This has been employed to immobilize the interesting enzyme beta-galactosidase from Aspergillus oryzae. The enzyme cannot be immobilized on just vinylsulfone activated support a pH values ranging from 5.0 to [read more]

Arturo Pérez2020-05-29T10:59:28+02:00Categories: publications2018|Tags: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical|