Biochemistry & Molecular Biology; Chemistry

Autores: Sabi, GJ; de Souza, L; Abellanas-Perez, P; Tardioli, PW; Mendes, AA; Rocha-Martin, J; Fernandez-Lafuente, R

Artículo.
Int. J. Biol. Macromol.. vol: 295. page: 0141-8130.
Fecha: MAR. 2025.
Doi: 10.1016/j.ijbiomac.2025.139667.

Resumen:
Eversa Transform (ETL) was immobilized on octyl agarose beads at two different enzymes loadings (1 mg/g and 15 mg/g) under 18 different conditions, including different pH values, buffers, additives (different solvents, [read more]

Autores: Abellanas-Perez, P; de Andrades, D; Alcántara, AR; Polizeli, MDTD; Rocha-Martin, J; Fernandez-Lafuente, R

Artículo.
Int. J. Biol. Macromol.. vol: 282. page: 0141-8130.
Fecha: DEC. 2024.
Doi: 10.1016/j.ijbiomac.2024.136812.

Resumen:
The focus of the present work is to find the optimal conditions for the activation of agarose beads with divinyl sulfone (DVS). The reactivity of the vinyl sulfone groups in the support was [read more]

Autores: Morellon-Sterling, R; Bolivar, JM; Fernandez-Lafuente, R

Artículo.
Int. J. Biol. Macromol.. vol: 220. page: 0141-8130.
Fecha: nov-01. 2022.
Doi: 10.1016/j.ijbiomac.2022.08.155.

Resumen:
The immobilization of ficin (a cysteinyl proteases) on vinyl sulfone agarose produced its almost full inactivation. It was observed that the incubation of the free and immobilized enzyme in beta-mercaptoethanol produced a 20 % of enzyme activity recovery, suggesting that [read more]

Autores: Sampaio, CS; Angelotti, JAF; Fernandez-Lafuente, R; Hirata, DB

Review.
Int. J. Biol. Macromol.. vol: 215. page: 0141-8130.
Fecha: AUG 31. 2022.
Doi: 10.1016/j.ijbiomac.2022.06.139.

Resumen:
In this review we have focused on the preparation of cross-linked enzyme aggregates (CLEAs) from lipases, as these are among the most used enzyme in bioprocesses. This immobilization method is considered very attractive due to preparation simplicity, [read more]

Autores: Arana-Pena, S; Carballares, D; Morellon-Sterling, R; Rocha-Martin, J; Fernandez-Lafuente, R

Artículo.
Int. J. Biol. Macromol.. vol: 199. page: 0141-8130.
Fecha: feb-28. 2022.
Doi: 10.1016/j.ijbiomac.2021.12.148.

Resumen:
The coimmobilization of lipases from Rhizomucor miehei (RML) and Candida antarctica (CALB) has been intended using agarose beads activated with divinyl sulfone. CALB could be immobilized on this support, while RML was not. However, RML was [read more]